3种药用石斛多糖的抗氧化活性比较研究  

Comparison of Antioxidant Activity of the Polysaccharides from Three Dendrobium Species

作　　者：王再花[1,2] 章金辉[1] 李杰[1] 徐晔春[1] 朱根发[1] 叶庆生[2] 

机构地区：[1]广东省农业科学院环境园艺研究所,广东省园林花卉种质创新综合利用重点实验室,广州510640 [2]华南师范大学生命科学学院,广东省植物发育生物工程重点实验室,广州510631

出　　处：《华南师范大学学报（自然科学版）》2015年第5期65-70,共6页Journal of South China Normal University(Natural Science Edition)

基　　金：广东省科技计划项目(2012B080701006);广东省农业建设与改革发展专项项目(粤农计〔2012〕138号);广东省农业科学院院长基金项目(201417)

摘　　要：比较了铁皮石斛、大苞鞘石斛和金钗石斛3种石斛茎段粗多糖、精多糖(除蛋白多糖)和多糖组分(过层析柱多糖分离组分)对DPPH·、·OH和O-23种自由基的离体抗氧化活性.结果表明:随着多糖纯度增强和质量浓度的提高,抗氧化效果均显著增强,在1.2 g/L时,处理效果最佳,铁皮石斛多糖组分(DOPP-I)、大苞鞘石斛多糖组分(DWPP-I)和金钗石斛多糖组分(DNPP-I)对DPPH·自由基的清除率均在75%以上,对·OH自由基的清除率在84%以上,且显著优于Vc(Vitamin c),但3种多糖组分间差异不显著;3种石斛多糖对O-2自由基的清除能力显著低于Vc,但DWPP-I的清除能力显著高于DOPP-I和DNPP-I.总之,大苞鞘石斛多糖组分DWPP-I的抗氧化能力略优于铁皮石斛和金钗石斛.The antioxidant activities of polysaccharides from three Dendrobium species （Dendrobium officinale, D. wardianum and D. nobile） were investigated in vitro. The results showed that antioxidant ability of Dendrobium pol- ysaccharides correlated well with the increasing of purities and concentrations, and the scavenging activities were the highest at 1.2 g/L. At this concentration, the scavenging rates by DOPP-I, DWPP-I and DNPP-I were above 75% for DPPH · radicals and above 84% for hydroxyl radicals （·OH）. It was insignificantly different among the three polysaecharides, but they were significantly higher than the control （Vitamin e）. The activity of DWPP-I to scavenge superoxide anion radicals （ O2^-） was significantly higher than DOPP-I and DNPP-I. Thus, antioxidant activity of polysaccharides DWPP-I （from D. wardianum） was higher than those from the other two Dendrobium poly- saecharides.

关 键 词：石斛 多糖 抗氧化活性 

分 类 号：S682.31[农业科学—观赏园艺]