气相色谱-质谱联用法检测牛乳中乙草胺的研究  

Determination of acetochlor in bovine milk by gas chromatography-mass spectrometry

作　　者：李琳瑶[1] 田成新 满朝新[2] 娄彬彬[1] 牛婕婷 张立东[1] 姜毓君[1,2] 

机构地区：[1]东北农业大学食品学院乳品科学教育部重点实验室,哈尔滨150030 [2]东北农业大学国家乳业工程技术研究中心,哈尔滨150030

出　　处：《中国乳品工业》2015年第9期34-37,共4页China Dairy Industry

基　　金：国家科技支撑计划课题(2013BAD18B11);黑龙江省杰出青年基金项目(JC201415);黑龙江省院所创新能力提升专项计划项目(YC13D005)

摘　　要：建立了牛乳中残留农药乙草胺的气相色谱-质谱联用（GC—Ms）检测方法。牛乳样品经乙腈振荡提取，氮吹浓缩，基质分散剂（基质分散剂由无水硫酸镁、PSA和Cleanert PestiCarbX；墨化炭黑组成）净化，气相色谱-质谱联用仪检测，外标法定量。结果表明，乙草胺在5-300μg／L质量浓度范围内线性关系良好，相关系数（R^2）为0．9991，平均回收率范围为93．42％-98．82％，相对标准偏差范围为2．00％-2．61％，乙草胺在4℃条件下30d内稳定性良好。该方法简单、灵敏、实用性强，适用于牛乳中乙草胺的检测分析。A gas chromatography-mass spectrometry （GC-MS） method was established for determination of acetochlor in bovine milk. The bovine milk was firstly extracted with acetonitrile by oscillation, concentrated under a gentle stream of nitrogen, then purified by matrix dis- persants （including anhydrous magnesium sulfate, PSA and Cleanert PestiCarb）. The acetochlor was detected by GC-MS and quantified by external standard method. The results showed the calibration curve had good linearity in the range of 5 to 300 μ g/L, linear correlation coeffi- cient was 0.9991, and average recoveries were in the range of 93.42% to 98.82% with a RSD of 2.00% to 2.61%. Acetochlor was stable with- in 30 days under the condition of 4℃. The method is sample, sensitive, practical and can be successfully applied for the determination oface- tochlor in bovine milk.

关 键 词：牛乳 乙草胺 气相色谱-质谱联用法(GC—MS) 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]