响应面对里氏木霉产纤维素酶液体发酵条件的优化  

Optimization of Culture Conditions for Cellulase Production of Trichoderma reesei by Response Surface Methodology

作　　者：王强强[1] 莫海飞 杨玉玲[1] 陈炼红[1] 伍红[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041

出　　处：《湖北农业科学》2015年第15期3735-3740,共6页Hubei Agricultural Sciences

基　　金：西南民族大学自然科学基金项目(2014NYB05)

摘　　要：采用单因素试验和响应面法对里氏木霉(Trichoderma reesei)Rut C-30产纤维素酶的液体发酵条件进行优化并以滤纸酶活力(FPA)作为响应值。结果表明,最优发酵条件为玉米芯粉3.42%,牛肉膏添加量1.70%,吐温-80添加量0.08%,初始pH 5.04,此条件下发酵液中的滤纸酶活力为12.10 U/m L,较未优化条件下得到的最高酶活力7.03 U/m L提高了72.12%。Single-factor tests and response surface methodology, with filter paper activity （FPA） as response value, were used to optimize the liquid-state fermentation medium for cellulase production by Trichoderrna reesei RutC-30.The results showed that the optimum medium was composed of corncob .powder 3.42%,beef extract 1.70% ,and Tween-80 0.08% with initial pH 5.04. Under this condition,the FPA reached to 12.10 U/mL and increased by 72.12%,compared with the maximum FPA （7.03 U/mL） before optimization.

关 键 词：里氏木霉(Trichoderma reesei) 玉米芯粉 纤维素酶 酶活 液体发酵 响应面 

分 类 号：TQ925[轻工技术与工程—发酵工程]