PTEN、p53和Survivin表达在胃癌组织中的意义  

Clinical significance of PTEN,p53 and Survivin expression in gastric carcinoma

作　　者：张海鹏 梁鹏锋 苗峰[3] 

机构地区：[1]陕西省中医医院普外科,西安710003 [2]陕西省渭南市中心医院普外科,714000 [3]陕西省西安市儿童医院新生儿外科,710056

出　　处：《检验医学与临床》2015年第18期2700-2702,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨PTEN、p53和Survivin表达在胃癌组织中的临床意义。方法选择2010-2013年陕西省中医医院收治的60例胃癌患者的胃癌标本作为A组,选择同期于陕西省中医医院手术切除吻合口切缘处的正常胃黏膜60例作为B组,另取不典型增生胃黏膜石蜡标本65例作为C组,采用免疫组织化学链霉亲和素方法检测Survivin、PTEN和p53基因在3组中的表达情况。结果胃癌组织中的Survivin、p53基因阳性率高于其他两组,差异具有统计学意义（P〈0.05）;Survivin基因的阳性表达和p53阳性呈正相关（P〈0.05）,和PTEN阴性率呈负相关（P〈0.05）;统计结果显示,3种基因在胃癌不同病理指标中的阳性表达差异有统计学意义（P〈0.05）。结论 Survivin、PTEN和p53基因异常表达和胃癌发生有密切的关系,其中PTEN与p53基因是判断胃癌生物学行为的重要指标,Survivin、PTEN、p53基因在胃癌发生及发展中有着相互协同的作用。Objective To investigate the expression and clinical significance of PTEN ,p53 and Survivin in gasric carcinoma tissue .Methods The gastric carcinoma samples in 60 cases of gastric carcinoma in the 2010 and 2013 in Shaanxi Provincial Hospital of Traditional Chinese Medicine were selected as the group A ,at the same period 60 samples of normal gastric mucosa in anastomosis stoma incisal edge in this hospital as the group B and 65 paraffin samples of atypical dysplasia gastric mucosa as the group C .The immunohistochemical streptavidin‐biotin method was adopted to detect the expression of Survivin ,PTEN and p53 gene in these three groups .Results The positive rate of Survivin and p53 gene in the gastric cancer tissue were higher than the that in the other two groups ,the difference was statistically significant（P〈 0 .05） ;the positive expression of Survivin gene was closely related with the p53 positive（P 〈 0 .05） ,showing the positive correlation ,and the negative correlated with PTEN negative rate （P〈0 .05） ;the statistic results showed that the positive expression of three genes had statistical difference among different pathological indicators of gastric cancer （P〈 0 .05） .Conclusion The abnormal expression of Survivin ,PTEN and p53 gene has the close relation with gastric cancer occurrence ,in which PTEN and p53 gene are the important indicaor for judging the biological behavior of gastric cancer ,Survivin ,PTEN and p53 gene have a mutual synergistic effect in the occurrence and development of gastric carcinoma .

关 键 词：SURVIVIN基因 PTEN基因 P53基因 胃癌 

分 类 号：R734.201[医药卫生—肿瘤]