油污土壤中脂肪酶产生菌的筛选及产酶条件优化  

Screening of Lipase-Producing Strain in Oil Field and Optimization of the Lipase Producing Conditions

作　　者：曲威[1] 邵蕾[1] 宋丽芬[1] 徐榕雪[1] 

机构地区：[1]中国农业大学烟台研究院,山东烟台264670

出　　处：《湖北农业科学》2015年第16期4002-4006,共5页Hubei Agricultural Sciences

基　　金：中国农业大学烟台研究院科研基金项目(YT201102)

摘　　要：为了获得脂肪酶产生菌,进而获得高酶活菌株,试验采集东营胜利油田被废油长期污染的土壤,通过罗丹明B平板法进行菌株分离筛选,并采用橄榄油乳化法测定脂肪酶酶活。经16S r RNA鉴定,确定为铜绿假单胞菌属,命名为Pseudomonas aeruginosa S8。并对该菌株的摇床培养产酶条件进行了初步研究,采用单因素试验和正交试验对S8菌株产脂肪酶的条件进行优化,得到最佳发酵培养基与条件为葡萄糖8 g/L,酵母粉8 g/L,硫酸铵6 g/L,花生油20 g/L,起始p H 7.0,接种量9%,温度30℃,发酵培养时间72 h,为工业化生产提供了出发菌株。In order to obtain lipase-producing strain of the bacteria,thus obtain high activity strains,the strain was screened from long waste-oil-pollutedsoil near Shenglin oil field in Dongying through Rhodamine B plate method and the lipase activity was determined with olive oil emulsification method. A strain S8 producing lipase was screened, which was identified as Pseudomonas aeruginosa by the identification of 16 S r RNA. The fermentation conditions of a lipase from S8 were determined by the single-factor experiment and orthogonal design. The result showed that the optimal medium composition for lipase production was as follows： glucose 8 g/L,yeast extract 8 g/L,（NH4）SO46 g/L, peanut oil 20 g/L. The optimal fermentation conditions were p H of 7.0, fermentation temperature of 30 ℃, inoculation amount of 9%, and fermentation time of 72 h,which provided foundation for the further study of lipase production fermentation.

关 键 词：脂肪酶 铜绿假单胞菌 筛选 鉴定 优化 

分 类 号：TQ925.6[轻工技术与工程—发酵工程] Q556.1[生物学—生物化学]