芭蕉叶挥发油的提取及其抗氧化活性研究  

Extraction and Antioxidant Activity of Essential Oil from Musa Acuminate Leaf

作　　者：李谷才[1] 陈容[1] 张儒[1] 唐勇军[1] 

机构地区：[1]湖南工程学院化学化工学院,湘潭411104

出　　处：《湖南工程学院学报（自然科学版）》2015年第3期59-61,69,共4页Journal of Hunan Institute of Engineering(Natural Science Edition)

基　　金：湖南省自然科学基金项目(13JJ3113);湖南省教育厅科研资助项目(14B043)

摘　　要：采用水蒸气蒸馏法提取了芭蕉叶挥发油,对挥发油进行了GC-MS分析,鉴定出29种成分.通过1,1-二苯基-2-三硝基苯肼(DPPH)法和β-胡萝卜素漂白法,测定了芭蕉叶挥发油的抗氧化活性;当挥发油浓度为0.30mg/mL时,对DPPH自由基的清除率达82.2%;在乳化脂质体系中,当挥发油浓度为50μg/mL时,β-胡萝卜素漂白抑制率达到92.0%.抗氧化活性研究表明:芭蕉叶挥发油具有较强的抗氧化活性.因此,芭蕉叶挥发油可以作为一种新型的天然抗氧化物质.The essential oil of Musa acuminateis extracted by hydrodistillation and twenty nine compounds in the oil are identified with gas chromatography-mass spectrometry （GC-MS）. The oil is subjected to a tioxidant activity screening by DPPH radical scavenging assay and β-carotene/linoleic acid bleaching test. When the content of the oil is 0.30 mg/mL, the scavenging efficiency for DPPH is 82.2 %. While for β- carotene bleaching system, its inhibitory percentage is 92.0% at the concentration of 50 μg/mL. The resuits obtained in this study suggest that the essential oil can serve as a novel source of natural antioxidants.

关 键 词：芭蕉叶 挥发油 气相色谱-质谱 抗氧化活性 

分 类 号：R284.1[医药卫生—中药学] O657.63[医药卫生—中医学]