不同提取方法对马齿苋水提物自由基清除和抗菌活性影响的研究  

Research on the Effect of Different Extraction Methods on Redicals Scavenging and Anti-microorganism Activities of the Aqueous Extract of Portulaca oleracea

作　　者：徐琳[1] 

机构地区：[1]山西省农业科学院农产品加工研究所,太原山西030031

出　　处：《安徽农业科学》2015年第27期53-54,66,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]比较分析了常规水提法和超声法2种方法获得的马齿苋水提物对自由基清除和抗菌活性影响。[方法]分别通过常规水提法和超声法获得了马齿苋水提物,然后将不同浓度的马齿苋水提物加入到反应液中,分别测试了它们的清除氧自由基、OH-自由基、DPPH自由基的活性,并分析比较了它们的抗菌活性。[结果]马齿苋水提物能够显著地清除上述3种自由基,同时对测试的5种菌(大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、酿酒酵母和黑曲霉)的生长均有一定的抑制作用;其中,超声法获得的马齿苋水提物在各测试浓度点的抗氧化抗菌活性均较常规水提法获得的马齿苋水提物强。[结论]超声法获得的马齿苋水提物具有较强的抗氧化抗菌活性。[ Objective ] The research aimed to compare and analyse the redicals scavenging and anti-microorganism activities of the aqueous extract of Portulaca oleracea from two extraction methods （conventional water extraction method and ultrasonic extraction method）. [ Method] Both conventional water extraction method and ultrasonic extraction method were used for obtaining the aqueous extract of Portulaca oleracea. Then, the different contents of the aqueous extract of Portulaca oleracea were added into reaction liquid, respectively. The radicals scavenging activity including O2- , OH - and DPPH were tested. In addition, their anti-microorganism activities were also compared and analysed. [ Result] The aqueous extract of Portulaca oleracea from two extraction ways could all obviously scavenge the O2- , OH - and DPPH radicals and inhibit the tested microorganism growth （bacterium coli, staphylococcus aureus, bacillus subtilis, saccharomyces cerevisiae, aspergillus niger）. Mo- reover, the aqueous extract of Portulaca oleracea from ultrasonic extraction method displayed the stronger antioxidant and anti-microorganism activities at the every tested content points than that from conventional water extraction method. [ Conclusion ] The aqueous extract of Portulaca oleracea from ultrasonic extraction method displayed the stronger antioxidant and anti-microorganism activities.

关 键 词：马齿苋水提物 常规水提法 超声法 自由基清除 抗菌活性 

分 类 号：S567.219[农业科学—中草药栽培]