丙烯酰胺对睾丸间质细胞R2C活性及孕酮合成功能的影响  

Effect of Acrylamide on the Viability and Progesterone Biosynthesis Function of Rat R2C Leydig Cells

作　　者：李名薇 孙建霞[2] 许伟[1] 邹飞雁[1] 白顺[1] 朱翠娟[1] 胡云峰[1] 焦睿[1] 吴实[1] 欧仕益[1] 冯梦鸽 白卫滨[1] 

机构地区：[1]暨南大学理工学院食品科学与工程系生命科学技术学院第一附属医院,广东广州510632 [2]广东工业大学轻工化工学院,广东广州510006

出　　处：《食品科学》2015年第17期247-251,共5页Food Science

基　　金：广东省高等学校优秀青年教师培养计划项目(Yq2013024);教育部"新世纪优秀人才支持计划"项目;国家自然科学基金青年科学基金项目(31201402;31201340)

摘　　要：目的:研究丙烯酰胺(acrylamide,AA)对体外培养的睾丸间质细胞R2C生长及孕酮合成功能的影响。方法:用浓度为0.25、0.5、0.75、1、2、4、6 mmol/L的AA作用于R2C细胞24 h,四甲基偶氮唑蓝(methyl thiazolyl tetrazolium,MTT)法获得IC25、IC50及IC75的AA作用浓度。用以上3种浓度的AA处理R2C细胞24 h,观察细胞形态。AA作用于R2C细胞4 h后,通过彗星实验(Comet assay)检测细胞DNA的损伤程度;放射免疫法(radioimmunoassay,RIA)测定AA处理R2C细胞4 h和24 h后的孕酮合成量。结果:AA能抑制R2C细胞活性,其IC25、IC50、IC75分别为1.140、1.925、3.250 mmol/L;3种浓度的AA能不同程度地影响R2C细胞生长形态;作用4 h对R2C细胞DNA有损伤作用;各浓度组作用24 h后均能降低R2C细胞的孕酮合成量。结论:AA能影响R2C细胞增殖及孕酮合成能力。Objective： To examine the effect of acrylamide （AA） on cell growth and progesterone synthesis in rat R2C cells. Methods： R2C cells were treated with AA at concentrations of 0.25, 0.5, 0.75, 1, 2, 4 and 6 mmol/L, respectively. Three inhibitory concentrations （IC25, IC50 and IC75） were determined by MTT （3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide） assay. Cell morphology was observed after being stimulated by the three inhibitory concentrations of AA for 24 h. DNA damage in R2C cells was measured by comet assay. The amounts of progesterone biosynthesis after being exposured to AA for 4 h and 24 h were detected by radioimmunoassay （RIA）. Results： AA could inhibit the cell viability. The IC25, ICs0 and IC75 were determined to be 1.140, 1.925 and 3.250 mmol/L, respectively. At these three concentrations, AA could affect cell morphology to different extends. DNA was significantly damaged at three concentrations of AA for 4 h, whereas the progesterone levels were significantly reduced after being exposed to AA for 24 h. Conclusion： AA can inhibit the cell growth and reduce the progesterone synthesis of R2C cells.

关 键 词：丙烯酰胺 R2C细胞 细胞活性 DNA损伤 孕酮 

分 类 号：Q26[生物学—细胞生物学]