牛屠宰厂来源的单增李斯特菌的耐药性研究  

Antimicrobial Susceptibility of Listeria monocytogenes from Beef Cattle Processing Plants

作　　者：冯晓慧[1] 张琳[1] 朱立贤[2] 

机构地区：[1]枣庄学院生命科学学院,山东枣庄277160 [2]山东农业大学食品科学与工程学院,山东泰安271018

出　　处：《枣庄学院学报》2015年第5期103-106,共4页Journal of Zaozhuang University

基　　金：山东省现代农业产业技术体系牛创新团队项目(项目编号:SDAIT-12-011-09)

摘　　要：了解牛屠宰厂来源的单增李斯特菌的耐药性情况.采用牛屠宰厂来源的的55株单增李斯特菌为研究对象,采用Kirby-Bauer法检测对16种抗生素的耐药情况.所检55株单增李斯特菌株对青霉素G、诺氟沙星、复方新诺明等不耐药,对头孢噻吩、多粘菌素B、头孢噻肟、依诺沙星的耐药率分别为1.82%、25.45%、36.36%、38.18%,耐3种以上抗生素的有44株,其中耐3种抗生素的27株(49.09%),耐4种抗生素的17株(30.90%).单增李斯特菌对4种抗生素存在耐药情况,并且存在不同程度的多重耐药情况的菌株,今后应加强对单增李斯特菌的污染情况和耐药性检测,以保证食品安全和人类健康.[ Objective]Listeria monocytogenes isolates from beef cattle processing plants were examined for antibiotic resist-ance.[Methods]Antimicrobial susceptibility to 16 kinds of antibiotics of 55 Listeria monocytogenes were determined by Kirby-Bauer method,respectively. [ Results]The 55 strains of Listeria monocytogenes were sensitive to penicillin G,norfloxacin, cotrimoxazole etc. Among the 55 Listeria monocytogenes isolates,1. 82% were resistant to cephalothin,followed by resistant to polymyxin B（25. 45%）,cefotaxime（36. 36%）,enoxacin（38. 18%）. 44 strains were resistant to more than 3 kinds of anti-biotic,among them 27 strains were resistant to 3 kinds of antibiotic（49. 09%）,17 strains were resistant to 4 kinds of antibiotic （30. 90%）.[ Conclusion]Listeria monocytogenes isolates were resistant to 4 antibiotics. Listeria monocytogenes appeared multidrug resistance in different degree. So the contamination and drug resistance of Listeria monocytogenes should be moni-tored to ensure food safety and human health in the future.

关 键 词：牛肉 单增李斯特菌 耐药性 

分 类 号：O657.32[理学—分析化学]