黄酒中抑制同型半胱氨酸诱导的大鼠血管平滑肌细胞增殖和迁移成分探讨  

Exploring the active ingredient of Chinese yellow wine which could inhibit the Hcy induced proliferation and migration of vascular smooth muscle cells

作　　者：孟立平[1] 周昌钻 郭艳[2] 翟小亚[3] 蒋承建[3] 李刚[3] 池菊芳[3] 郭航远[1,3] 

机构地区：[1]温州医科大学第一临床医学院,浙江温州325000 [2]浙江中医药大学,杭州310000 [3]绍兴市人民医院心内科,浙江绍兴312000

出　　处：《中国应用生理学杂志》2015年第5期437-442,共6页Chinese Journal of Applied Physiology

基　　金：浙江省自然科学基金项目(LY14H020002);浙江省公益技术研究社会发展项目(2012C33040);绍兴市科技局项目(2013B70072)

摘　　要：目的：探索黄酒中具有抑制同型半胱氨酸（Hcy）诱导的大鼠血管平滑肌细胞（VSMCs）增殖和迁移作用的活性成分。方法：SD大鼠主动脉VSMCs原代细胞培养鉴定,取4-7代细胞用于实验。VSMCs分为control组、Hcy（浓度为1 mmol/L）组、低聚糖组（Hcy＋黄酒低聚糖）、多肽组（Hcy＋黄酒多肽）、多酚组（Hcy＋黄酒多酚）、酒精组（Hcy＋酒精）、黄酒组（Hcy＋黄酒）共7组。MTT法检测各组VSMCs的增殖情况;划痕法和Transwell法检测各组VSMCs的迁移情况;Western blot法检测各组VSMCs中基质金属蛋白酶-2/9（MMP-2/9）、基质金属蛋白酶的组织抑制剂-2（TIMP-2）的表达情况;明胶酶谱检测各组VSMCs中MMP-2/9的活性。结果：与control组相比,Hcy组VSMCs增殖迁移增加、MMP-2/9表达和活性增加（P〈0.01）。与Hcy组相比,多肽组、多酚组、黄酒组VSMCs增殖迁移减少、MMP-2/9表达和活性减少（P〈0.05）。各组之间TIMP-2的表达没有显著差异。结论：黄酒中的多肽类和多酚类成分具有抑制Hcy诱导的VSMCs增殖和迁移的作用。Objective： To explore the active ingredients in the Chinese yellow wine could inhibit the proliferation and migration of rat vas- cular smooth muscle cells induced by homocysteine （Hcy）. Methods： The primary culture and identification of rat vascular smooth muscle cells （VSMCs） was conducted, and the VSMCs in passage4-7 were used in the following experiments. The VSMCs were divided into 7 groups： control, Hcy（1 mmoL/L）, Hcy ＋ oligosaccharide, Hcy ＋ polypeptides, Hcy ＋ polyphenols, Hcy ＋ alcohol, Hcy ＋ Chinese yellow wine and were given the corresponding treatment. The pmhferation of VSMCs was determined by MTr. Transwell chambers and wound healing were employed to test the migratory ability of VSMCs. Western blot and gelatin zymography were used to investigate the expressions and activities of metal matrix proteinase 2/9 （MMP-2/9） and tissue inhibitor of metalloproteinase 2 （TIMP-2） in VSMCs of each group. Results： Compared with control group, the proliferation, migration and the expression and activity of MMP-2/9 of VSMCs were significantly increased in the VSMCs of Hcy group（ P 〈 0.01 ）. Compared with Hcy group, the proliferation, migration and the expression and activity of MMP-2/9 of VSMCs were significantly decreased in the VSMCs of pelypeptides group, pelyphenols group and Chinese yellow wine group. However, the expression of TIMP-2 among each group had no significant difference. Conclusion： Polypeptides and polyphenols in the Chinese yellow wine could inhibit the proliferation and migration of VSMCs induced by Hcy.

关 键 词：大鼠血管平滑肌细胞 黄酒 多肽 多酚 基质金属蛋白酶 同型半胱氨酸 大鼠 

分 类 号：R543.3[医药卫生—心血管疾病]