引入血红素生物合成基因对褐黄孢链霉菌产纳他霉素的影响  

Effect of inducing heme gene on natamycin production by Streptomyces gilvosporeus

作　　者：杨天[1,2] 殷瑜[2] 仲茜[1] 戈梅[2] 钱秀萍[1] 

机构地区：[1]上海交通大学药学院,200240 [2]上海来益生物药物研究开发中心有限责任公司,201203

出　　处：《中国医药生物技术》2015年第5期416-419,共4页Chinese Medicinal Biotechnology

摘　　要：目的考察引入血红素合成关键基因对褐黄孢链霉菌生产纳他霉素的影响。方法运用PCR方法扩增相关血红素合成基因hem A1、hem B、hem E,使用属间接合转移的方法转化入褐黄孢链霉菌HCCB13086,获得引入血红素基因的工程菌株;通过摇瓶发酵考察突变菌株对载氧能力及纳他霉素产量的影响。结果成功构建3个引入血红素合成关键基因的菌株,3株菌在相对高氧条件下生长及产抗能力均与对照组相当,而在相对低氧条件下纳他霉素产量均较对照菌株提高,其中引入hem A1血红素基因的菌株提高最为显著,较对照组的纳他霉素产量提高64%,达到8.52 g/L。结论引入血红素合成基因可提高菌体对氧的利用,促进菌体生长和代谢产物的增加。Objective To investigate the effect of inducing key heme biosynthetic genes on natamycin production by Streptomyces gilvosporeus. Methods In order to induce heme genes, genes hemA 1, B, E were PCR amplified and transformed into Streptomyces gilvosporeus HCCB 13086 by intergeneric conjugation transformation. The strain growth and natamycin yields of mutant strains under different dissolved oxygen levels were investigated through shaking flask fermentation. Results Three strains carrying key heme biosynthetic genes were successfully constructed. The natamycin yield of inducing hemA 1 mutant was equal to the wild strain when fermented in relative high dissolved oxygen level flasks, while the yield was increased by 64%, reaching 8.52 g/L, under relatively low oxygen. Conclusion Inducing heme biosynthetic gene can raise the oxygen utilization and promote the strain growth and metabolite accumulation.

关 键 词：纳他霉素 血红素 褐黄孢链霉菌 载氧能力 

分 类 号：R379[医药卫生—病原生物学]