黑木耳多糖对内毒素诱导急性肺损伤大鼠肺组织的保护作用  

Protective effect of Auricularia auricular-judae polysaccharide on pulmonary tissues of rats with LPS-induced acute lung injury

作　　者：高阳[1] 刘斌[1] 哈尼再尔.热合曼 李转运[1] 孙湛[2] 

机构地区：[1]新疆医科大学临床医学院,新疆乌鲁木齐830054 [2]新疆医科大学基础医学院,新疆乌鲁木齐830054

出　　处：《中国病理生理杂志》2015年第9期1704-1708,1714,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81260454);新疆维吾尔自治区大学生创新实验项目(No.CX2014021)

摘　　要：目的:探讨黑木耳多糖对LPS诱导急性肺损伤大鼠肺组织的保护作用及其机制。方法:将健康SD大鼠随机分为对照组、LPS组、地塞米松组以及黑木耳多糖低、中、高浓度组,根据分组,分别给予生理盐水或不同浓度黑木耳多糖预防性灌胃7 d,第8天腹腔注射生理盐水或LPS(8 mg/kg),地塞米松组在给予LPS后腹腔注射地塞米松(3 mg/kg)。造模12 h后于腹主动脉取血,并制肺组织匀浆和肺泡灌洗液。检测支气管肺泡灌洗液中蛋白含量、肺湿/干重比、髓过氧化物酶(MPO)、总抗氧化能力(T-AOC)、总超氧化物歧化酶(T-SOD)、一氧化氮合酶(NOS)、丙二醛(MDA)等指标,做组织切片HE染色并进行肺损伤评分。结果:应用黑木耳多糖干预后,急性肺损伤大鼠支气管肺泡灌洗液中蛋白含量明显下降,肺湿/干重比值降低;大鼠肺组织中MPO、NOS活性及MDA含量较LPS组降低,T-AOC含量和T-SOD活性较之升高;肺组织病理改变减轻,肺损伤指数下降。结论:黑木耳多糖具有保护LPS损伤大鼠肺组织的作用,其机制可能与其抗氧化作用有关。AIM：To investigate the effects of Auricularia auricular-judae polysaccharide （ AAP） on pulmonary tissues of rats with LPS-induced acute lung injury （ALI） and its mechanisms.METHODS： Adult Sprague-Dawley rats were randomly divided into control group , LPS group, low-dose AAP group, middle-dose AAP group, high-dose APP group, and dexamethasone group.The rats were injected with LPS （8 mg/kg, ip） to induce ALI.The rats in the AAP groups were treated with AAP for 7 d before the induction of ALI .The protein concentration in the bronchoalveolar lavage fluid （BALF） was measured.The lung edema degree was measured by detecting the wet /dry weight ratio.The myeloper-oxidase （ MPO） , total antioxidant capacity （ T-AOC） , total superoxide dismutase （ T-SOD） , nitric oxide synthase （ NOS） and malondialdehyde （ MDA） levels were determined .The pathological changes of the lung tissues were evaluated by HE staining.RESULTS：Treatment with AAP significantly improved LPS-induced lung pathological changes , attenuated the protein concentration in the BALF and wet/dry weight ratio , inhibited the activities of MPO and NOS , reduced MDA level and increased the activities of T-AOC and T-SOD.CONCLUSION： AAP protects against LPS-induced acute lung injury in rats.

关 键 词：黑木耳多糖 脂多糖 急性肺损伤 抗氧化作用 

分 类 号：R363.1[医药卫生—病理学]