狂犬病病毒RT-PCR检测方法的建立  

Method of RT-PCR for Detection of Rabies Virus

作　　者：周萍 吴晓薇[2] 洪洁心[2] 刘志玲[2] 高志强 郭霄峰[4] 

机构地区：[1]浙江省慈溪市崇寿镇人民政府,浙江慈溪315334 [2]广东出入境检验检疫技术中心,广东广州510623 [3]北京检验检疫技术中心,北京100000 [4]华南农业大学兽医学院,广东广州510642

出　　处：《广东畜牧兽医科技》2015年第5期41-46,共6页Guangdong Journal of Animal and Veterinary Science

基　　金：国家质检总局科技计划项目(2012IK005)

摘　　要：为建立一种检测狂犬病病毒的方法,根据Gen Bank已公布的狂犬病病毒核蛋白N基因序列,设计并合成一对特异性的引物,以兽用疫苗株HEP-Flury为阳性毒株建立了RT-PCR检测方法。对建立的RT-PCR方法做灵敏度、特异性、重复性及稳定性试验,结果显示该方法的灵敏度可以达到20 FFU/m L,可以将狂犬病病毒与犬瘟热病毒和犬细小病毒区别开来。该方法重复性好,稳定可靠。To establish the method of RT-PCR for detection of Rabies virus,a pair of primers were designed based on nucleoprotein sequences of Rabies virus that published in GenBank. RNA extracted from Rabies virus vaccine HEP-Rlury was as standard positive control. The results showed that the method could distinguish Rabies virus from canine distemper virus,canine parvovirus.The sensitivity could attained 20 FFU/mL and the stability was good.

关 键 词：狂犬病病毒 N蛋白基因 RT-PCR 检测方法 

分 类 号：S852.655[农业科学—基础兽医学]