改性玉米芯固定化脂肪酶研究  

Research of Modified Corncobs Immobilized Lipase Conditions

作　　者：廖晓峰[1] 于荣[1] 梁华正[1] 张吉祥[1] 

机构地区：[1]东华理工大学,江西南昌330038

出　　处：《广州化工》2015年第18期94-97,共4页GuangZhou Chemical Industry

摘　　要：以玉米芯为原料,分别用饱和氢氧化钾和2%磷酸浸泡处理,采用三羟甲基氨基甲烷作为活化剂,活化温度60℃,活化时间3.5~4 h,活化剂浓度8%。利用改性玉米芯对脂肪酶固定化。实验结果表明：活化玉米芯载体固定化脂肪酶的最佳工艺条件为：液态酶/载体比为11.5∶1,酶固定化时间10 h,固定化温度35℃,所用缓冲液p H 6.8。Using corncobs as raw material, soaking by potassium hydroxide and phosphoric acid, separately, with three hydroxymethyl amino methane （ THAM） as the activator, activation temperature of 60℃, activation time of 3. 5~4 h, and the concentration of the activator of 8%, lipase immobilization was studied. The results presented that the best immobilized conditions of enzyme liquid/carrier ratio was 11. 5∶1, pH 6. 8 of buffer solution, and 10 h fixed time with 35 ℃.

关 键 词：改性玉米芯 固定化脂肪酶 酶活力 

分 类 号：O657.31[理学—分析化学]