树突状细胞激活介导副干酪乳杆菌L9对CD4^+T细胞的分化作用研究  

Dendritic Cells Mediates the Lactobacillus Paracasei L9-Induced CD4^+T Cell Differentiation

作　　者：杨景[1] 刘松玲[1] 葛绍阳[1] 刘力[1] 桑跃 武永超[1] 张建铭 刘治麟 赵亮[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京市高等学校畜产品工程研究中心 [2]江西鸿燕健康科技有限公司 [3]北京和益源生物技术有限公司

出　　处：《中国乳业》2015年第9期65-69,共5页China Dairy

基　　金：中央在京高校重大成果转化项目"益生菌生产关键技术科技成果转化"

摘　　要：为研究副干酪乳杆菌L9(L9)诱导CD4+T细胞分化的作用机制,以骨髓源树突状细胞(BMDCs)为模型,通过酶联免疫和流式细胞术分析L9对BMDCs调节性细胞因子分泌以及CD103表达的影响;利用L9诱导后的BMDCs与小鼠脾CD4+T细胞联合培养,分析调节性T(Foxp3+Treg)细胞的比例。结果表明,与空白组相比,L9的干预能够显著促进BMDCs中白介素(IL-10)和转化生长因子β(TGF-β)的分泌以及CD103的表达(P<0.05),并呈现浓度依赖效应;同时,经L9诱导后的BMDCs,显著提高了CD4+T细胞中Foxp3+Treg细胞的比例(P<0.05)。L9通过调节BMDCs中调节性细胞因子的分泌以及CD103的表达,促进CD4+T细胞向Foxp3+Treg细胞分化。To investigate the mechanism of Lactobacillus paracasei L9 （L9） -regulated CD4＊T cell differentiation, dendritic cells （ DCs ） generated from bone marrow of BALB/c mouse were used in this study. The regulatory cytokines production and CD103 expression in bone marrow dendritic cells （BMDCs） were analyzed by ELISA and flow cytometry. The percentage of regulatory T （ Treg ） cells in CD4^＋T cell cocultured with L9-simulated BMDCs was analyzed. The results showed that, compared with the blank control, L9 significantly increased the production of IL-IO and TGF-β from BMDCs, as well as the expression of CD103 （ P 〈 0.05 ） , in dose-dependent effect. Furthermore, the number of the CD25^＋Foxp3^＋ cells was increased （ P 〈 0.05 ） in CD4^＋ T cells cocultured with L9-simulated BMDCs. These results demonstrated that L9 increased regulatory cytokines production and CD103 expression in BMDCs, which induced the conversion of the Foxp3^＋Treg cells from CD4^＋T cells.

关 键 词：副干酪乳杆菌L9 树突状细胞 T调节细胞 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]