基于新型乙酰胆碱酯酶传感器的有机磷农药检测  

Detection of organophosphorus pesticide based on novel acetylcholinesterase biosensor

作　　者：王静静[1] 赵凤娟[2] 卫敏[1] 

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001 [2]深圳出入境检验检疫局食品检验检疫技术中心深圳市食品安全检测技术研发重点实验室,广东深圳518045

出　　处：《粮油食品科技》2015年第5期77-81,共5页Science and Technology of Cereals,Oils and Foods

基　　金：河南省高等学校青年骨干教师项目资助(2013GGJS-073);河南省教育厅项目(14B550014);河南省产学研合作项目(142107000023)

摘　　要：制备了空心碳球—离子液体1-丁基-3-甲基咪唑磷钨酸盐（HCS-[Bmim]3PW12O40）修饰玻碳（GC）电极,采用交联法将乙酰胆碱酯酶（ACh E）固定在修饰电极表面,获得ACh E/HCS-[Bmim]3PW12O40/GC传感器。在最优条件下,用该传感器对有机磷农药久效磷、克百威标准品分别进行检测,久效磷的线性范围为8.00×10^-10~8.00×10^-6g/L,克百威的线性范围是1.00×10^-10~1.00×10^-7g/L。按抑制率10%计算,久效磷和克百威的检出限分别为7.58×10-11g/L和6.76×10-11g/L。所制备的ACh E/HCS-[Bmim]3PW12O40/GC传感器灵敏度高和稳定性好,为有机磷农药的分析提供了一种有前景的工具。Hollow carbon spheres - ionic liquid 1 - butyl -3 - methyl imidazole phosphotungstate （ HCS - [ Bmim] 3PW12040 ） was prepared to modify glassy carbon （GC） electrode. Acetylcholinesterase （ACHE） was immobilized on the electrode surface by cross -linking reaction to obtain AChE/HCS - [ Bmim] 3PWI2040/GC sensor. Under the optimal condition, the organophosphorus pesticides, monocro- tophos and carbofuran, were detected by the biosensor. The linear range of monocrotophos was 8.00 ×10^-10 -8.00 ×10^-6 g/L, and that of earbofuran 1.00 ×10^-10 - 1.00 ×10^-7 g/L, with the detection limits of 7.58 ×10^-11 g/L and 6.76 ×10^-11 g/L, respectively, calculated by inhibition rate 10%. The biosensor exhibited high sensitivity and acceptable stability, and could be a promising tool for analysis of organophosphorus pesticides.

关 键 词：空心碳球—离子液体1-丁基-3-甲基咪唑磷钨酸盐 玻碳电极 乙酰胆碱酯酶 氯化乙酰胆碱 久效磷 克百威 

分 类 号：TS207.3[轻工技术与工程—食品科学]