柱色谱分离纯化五倍子没食子酸  

Separation and Purification of Gallic Acid in Galla chinensis by Chromatography

作　　者：廖格[1,2,3] 卲元元 熊硕[1,2] 邓娣 肖文军[1,2] 

机构地区：[1]湖南农业大学国家植物功能成分利用工程技术研究中心 [2]湖南省植物功能成分利用协同创新中心,长沙410128 [3]湖南农业大学园艺园林学院植物资源工程系,长沙410128 [3]海南大学园艺园林学院园艺系,海口570228

出　　处：《中国食品学报》2015年第7期131-138,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：“十二五”国家科技支撑计划项目(2011BAD10B00);国家农业科技成果转化资金项目(2011GB2D200010)

摘　　要：目的:优化筛选分离纯化五倍子没食子酸的最优树脂及其工艺参数。方法:以五倍子水提取液为原料,比较NKA-2、LS303、HZ816、HP-20、XAD-16、HPD700、HPD500、S-8、AB-8、D101、D301 11种树脂对五倍子没食子酸的静态吸附与解吸效果,筛选最优树脂。采用单因素试验、正交试验与验证性试验,优化最优树脂动态吸附与解吸五倍子没食子酸的工艺参数。结果:NKA-2树脂为最优树脂,静态吸附量与解吸率分别达到94.856 mg/g和89.64%;当主要考虑没食子酸纯度时,其最优动态吸附与解吸工艺参数是:上样质量浓度7 mg/m L,上样体积7BV,上样流速2 BV/h,洗脱剂乙醇体积分数70%,洗脱体积2.5 BV,洗脱流速3 BV/h,所获五倍子没食子酸的纯度、得率分别为90.97%和78.84%;当主要考虑没食子酸得率时,最优动态吸附与解吸工艺参数为上样质量浓度5 mg/mL,上样体积5 BV,流速3 BV/h,洗脱剂乙醇体积分数70%,洗脱体积2.5 BV,洗脱流速2 BV/h,所获五倍子没食子酸的得率、纯度分别为85.04%和87.10%。结论:NKA-2大孔吸附树脂是一种可应用于五倍子没食子酸分离纯化的较好树脂。Objective： Studies on separation and purification of gallic acid from Galla chinensis by different resins.Methods： The research compared the absorption capacity and desorbtion effect with different concentration of alcohol of 11 kinds of resins： NKA-2、 LS303、 HZ816、 HP-20、 XAD-16、 HPD700、 HPD500、 S-8、 AB-8、 D101、 D301, and the best processing parameters were optimized based on the adsorption and desorption results. Results： NKA-2 was the best one. An optimum purification parameter with sample concentration 7 mg/m L, sample volume 7 BV, flow rate 2 BV/h, elution ethanol concentration 70%, elution volume 2.5 BV, flow rate 3 BV/h was built and applied to separate gallic acid, in this situation the result was obtained that the purity is 90.97% and the yield is 78.84%. An optimum yield parameter with sample concentration 5 mg/mL, sample volume 5 BV, flow rate 3 BV/h, elution ethanol concentration 70%,elution volume 2.5 BV, flow rate 2 BV/h was built and applied to separate gallic acid, in this situation the result was obtained that the yield is 85.04% and the purity is 87.10%. Conclusion： NKA-2 macroporous resin has a good purification effect in gallic acid in Galla chinensis.

关 键 词：五倍子 没食子酸 柱色谱 大孔吸附树脂 分离纯化 

分 类 号：O652.6[理学—分析化学] R284[理学—化学]