芘荧光探针法研究燕麦β-葡聚糖溶液中的疏水微区  

Study on the Hydrophobic Microdomain in Solution of Oat β-Glucan Using Pyrene Fluorescence Probe

作　　者：黄回阳[1] 吴小燕[1] 李琳琳[1] 戴巧玲[1] 张如[1] 吴佳[1] 

机构地区：[1]福州大学生物科学与工程学院,福州350116

出　　处：《中国食品学报》2015年第7期225-229,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31101224);福建省自然科学基金项目(2013J05049)

摘　　要：采用荧光探针技术,通过测定芘荧光发射峰强度的比值I1/I3,研究β-葡聚糖在水溶液中疏水微区的变化情况。试验结果表明,β-葡聚糖溶液在极小质量浓度下基本不形成疏水微区,当质量浓度超过一定值时开始大量形成疏水微区,I1/I3值迅速下降。在相同质量浓度条件下,相对分子质量较小的β-葡聚糖所形成的疏水微区数量也较少。尿素能够破坏疏水微区的形成,氯化钠能够促进疏水微区的形成。In this paper, fluorescent probe technique was used to study the changes of hydrophobic microdomain inβ-glucan aqueous solution by measuring the pyrene fluorescence emission intensity ratio I1/I3. The results show that, hydrophobic microdomain is basically not formed in diluted β-glucan solution. When the concentration exceeds a certain value, hydrophobic microdomains start to form in large quantities and the value of I1/I3 decreases rapidly. At the same concentration, the lower molecular weight β-glucan would form less hydrophobic microdomains. Urea can damage the formation of hydrophobic microdomain, while sodium chloride can promote the formation of hydrophobic domain.

关 键 词：Β-葡聚糖 疏水微区 芘荧光探针 

分 类 号：O657.3[理学—分析化学] TS210.7[理学—化学]