一种水溶性壳聚糖的制备及用于微囊化细胞培养的研究  

Preparation of water-soluble chitosan and application in microencapsulated cell culture

作　　者：郑国爽[1,2] 郑会珍[1,2] 谢红国[1] 刘袖洞[3] 于炜婷[1] 马小军[1] 

机构地区：[1]中国科学院大连化学物理研究所生物医用材料研究组,辽宁大连116023 [2]中国科学院大学,北京100049 [3]大连大学环境与化学工程学院,辽宁大连116622

出　　处：《功能材料》2015年第20期20078-20082,共5页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(51103157,21276033);国家重点基础研究发展计划(973计划)资助项目(2012CB720801)

摘　　要：为了提高壳聚糖的水溶性,通过一种简单的均相介质中N-乙酰化方法制备了水溶性壳聚糖。利用傅里叶红外光谱仪、核磁共振仪和X射线衍射仪表征了水溶性壳聚糖的脱乙酰度(DD)、官能团结构和结晶状态,进一步分析了其水溶机理。初步验证了水溶性壳聚糖用于微囊化细胞培养的可行性,结果表明,水溶性壳聚糖脱乙酰度为56%,在乙酰化过程中,晶体的结晶度降低而形成无定形结构是其水溶的主要原因;细胞在水溶性壳聚糖制备的微胶囊中保持良好的活性,在培养过程中逐渐聚集成团生长。To improve the water-solubility of chitosan,a simple N-acetylation in homogeneous medium was developed to prepare soluble chitosan.The degree of deacetylation(DD),structure of functional groups and crystalline state of soluble chitosan were characterized by FT-IR,NMR and XRD,which helped to analyze the water-solubility mechanism.Moreover,microcapsules were prepared with soluble chitosan and tested for immobilized cell culture.The results showed that DD of soluble chitosan was 56%,and the formation of amorphous structure due to decrease of degree of crystallinity was the main reason to get soluble chitosan.Finally,it was observed that cells in microcapsules with soluble chitosan kept high activity and grew gradually to form cell aggregation during culture process.These results demonstrated that the water-soluble chitosan was feasible for preparing microcapsules and applying in cell culture.

关 键 词：水溶性壳聚糖 NG乙酰化 脱乙酰度 微胶囊 细胞培养 

分 类 号：TB332[一般工业技术—材料科学与工程] O63[理学—高分子化学]