响应面法优化粪产碱菌Alcaligenes faecalis DL-08产纤维素酶培养工艺  

Cellulase production process of Alcaligenes faecalis DL-08 by response surface optimization

作　　者：杜冰[1,2] 郑来久[1,2] 魏取福[1] 

机构地区：[1]江南大学纺织服装学院,无锡214122 [2]大连工业大学辽宁省清洁化纺织重点实验室,大连116034

出　　处：《工业微生物》2015年第5期7-12,共6页Industrial Microbiology

基　　金：辽宁省博士启动基金项目(20111069)

摘　　要：利用粪产碱菌Alcaligenes faecalis DL-08,进行产纤维素酶培养,在Plackett-Burman设计基础上,通过Design-Expert中心组合实验,建立了培养条件优化的二次回归模型,并通过响应曲面分析,确定了最佳培养条件。由响应面分析结果可知,产纤维素酶条件:温度37.98℃,碳氮比1∶1,接种量2%,初始p H 7,时间24 h。在此条件下,预测值OD520为1.237。经3组平行试验,OD520平均值为1.229,与模型预测值基本一致,且与未优化条件相比,纤维素酶活力提高了5.6%,可达1 303.2 U/m L。Alcaligenes faecalis DL-08 was used to produce cellulase by Plackett-Burman design, and quadratic regression model of culture conditions was established by Design-Expert Center Combination. Then the response surface analysis was used to determine the optimum culture conditions, and the results obtained were as follows ： temperature 37.98℃, C/N ratio 1 ： 1, inoculation size 2%, pH 7 and time 24 h. The predicted maximal 0D520 was 1. 237. After thrce sets of parallel experiments, the average 0D520 was 1. 229, indicating that the actual values and predicted values were basically the same, and the cellulase activity increased by 5.6% , up to 1 303.2 U/mL.

关 键 词：响应面法 粪产碱菌 纤维素酶 二次回归模型 

分 类 号：TQ925[轻工技术与工程—发酵工程]