脑卒中后中枢性疼痛相关血浆氨基酸筛选  

Screening out the Plasma Amino Acids Related to Central Poststroke Pain

作　　者：吴芳玲[1] 杨坚[1] 赵明雷[1] 刘海明[2] 高宁沁[1] 舒晓燕[1] 

机构地区：[1]上海市徐汇区中心医院康复科,200031 [2]中国科学院上海临床中心实验室

出　　处：《医学研究杂志》2015年第9期115-118,共4页Journal of Medical Research

基　　金：上海市徐汇区中心医院基金资助项目(2012XHCH14)

摘　　要：目的观察脑卒中后中枢性疼痛（central poststrokepain，CPSP）患者9种相关血浆游离氨基酸浓度，筛选与CPSP发病相关的氨基酸递质。方法112例脑卒中后2—8周患者根据是否伴发CPSP分为CPSP组30例、无CPSP（nCPSP）组82例，采用高效液相色谱-质谱分析技术测定了所有患者及36例正常对照的9种血浆游离氨基酸谱。结果CPSP组及nCPSP组的谷氨酸、谷氨酰胺均明显升高（P〈0．05），牛磺酸、天冬酰胺均明显降低（P〈0．05），nCPSP组的天冬氨酸、甘氨酸、酪氨酸、苯丙氨酸明显降低（P〈0．05），CPSP组色氨酸明显降低（P〈0．05）。结论血浆兴奋性氨基酸水平的升高和抑制性氨基酸水平的降低可能与CPSP的发病并无关联，但作为体内儿茶酚胺、5-羟色胺类递质的前体，酪氨酸、苯丙氨酸、色氨酸，则可能参与了CPSP的发病机制。Objective To filter the amino acid neurotransmitters that are associated with central poststroke pain （CPSP） , the plasma concentrations of 9 relative free amino acids were evaluated in the stroke patients. Methods CPSP group with 30 patients and nCPSP （ non - CPSP） group with 82 patients were selected out of 112 patients having stroke 2 - 8 weeks ago, whose plasma concentrations of the 9 free amino acids were measured with HPLC - MS in contrast to those of 36 controls. Results Glutamate and glutamine were observed significantly elevated both in CPSP and nCPSP groups （P 〈 0.05 ） , while the taurine and asparagines dramatically decreased in these two groups （P 〈 0.05）. Notably decrease was found in the concentrations of aspartic acid, tyrosine, glycine and phenylalanine in nCPSP group （P 〈 0.05） and tryptophan in CPSP group （P 〈 0.05）. Conclusion According to the observations, there is no clear evidence indicating the correlation between the onset of CPSP and the increase of excitatory amino acid or the decrease of inhibitory amino acid. However, as the raw materials of catecholamine and 5 -hydroxytryptamine, tyrosine, phenylalanine and tryptophan may be associated with the pathogenesis of CPSP.

关 键 词：脑卒中 卒中后中枢性疼痛 氨基酸 

分 类 号：R74[医药卫生—神经病学与精神病学]