利用生物信息学技术预测副溶血弧菌噬菌体VPp1内溶素基因及其裂解机制  

Bioinformatic prediction of endolysin gene function and its lysis mechanism in Vibrio Parahaemolyticus phage VPp1

作　　者：金延秋[1] 庄松娟 林洪[1] 王静雪[1] 孙翌 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]青岛市食品药品检验研究院,山东青岛266071 [3]青岛市城市排水监测站,山东青岛266002

出　　处：《中国渔业质量与标准》2015年第5期67-71,共5页Chinese Fishery Quality and Standards

基　　金：海洋水产食品加工技术研发与产业化示范(2012BAD28B05);现代农业产业体系(CARS-50)

摘　　要：通过对副溶血弧菌噬菌体VPp1全基因组分析,利用相似性比对数据库BLAST和序列模体数据库,初步推定该基因组的ORF31编码内溶素,预测该开放式阅读框编码一种糖苷水解酶。预测内溶素不含信号肽、跨膜区域。从生物信息学角度推定噬菌体VPp1内溶素的裂解机制可能是联合孔蛋白协同作用裂解细菌肽聚糖。本研究为进一步揭示内溶素生物学特性提供基础数据。According to the genetic analysis of Vibrio Parahaemolyticus phage VPpl genome, it was proposed primarily that ORF31 was predicted to encode phage endolysin by BLAST and functional analysis. ORF31 was predicted to be a kind of glucoside hydrolase without signal peptide and transmembrance domain. Furthermore, holin genes was not de- tected, which might due to the diversity of holin sequences. It was speculated that phage VPpl endolysin might need the help of holin in bacteria - lysing process. This study provides a scientific basis for the further verifying the biologi- cal characteristics of endolysin gene in VPpl genome.

关 键 词：噬菌体 副溶血弧菌 内溶素 蛋白结构 生物信息分析 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程] Q811.4[轻工技术与工程—食品科学与工程]