低桔霉素且产Monacolin K红曲菌株的筛选与鉴定  

Screening and identification of Monacolin K-production Monascus with low citrinin

作　　者：刘颖[1,2] 林风[1,2] 郑军荣 陈利丁[1] 高振云[1] 李志强[1] 王海龙[1,2] 王焌翔[1] 吴丽云 

机构地区：[1]福建省微生物研究所 [2]福建省新药(微生物)筛选重点实验室,福建福州350007

出　　处：《福建农林大学学报（自然科学版）》2015年第5期456-461,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省重点项目(2013N0007);福建省公益类科研院所专项(2014R1009-6);福建省科技平台建设(2014Y2001);福州区校(院所)科技合作项目(2014X02);福州仓山区科技创新公共服务平台(2015K03)

摘　　要：对福建古田地区不同来源的红曲样品进行分离纯化,结合固态发酵进行定向筛选,并通过菌落形态特征观察、分子生物学手段进行初步的分类鉴定.获得一株功能红曲菌株NW3-2,其固态发酵产品中桔霉素的含量低于国家有关有毒物质的最低检测标准,酸式和内酯式Monacolin K含量分别为3.20和2.47 mg·g-1.The samples from Gutian,Fujian were isolated and purified for Monacolin K yield Monascus strains with low citrinin level. The Monascus strains were selected directly by solid fermentation,and preliminarily catalogued by morphologic analysis combined with molecular identification. A new functional Monascus strain NW3-2 was obtained. The content of toxic citrinin was lower than national standards,and the contents of hypolipidemic ingredient of acid form and lactones form Monacolin K were 3.20 and 2.47 mg·g-1,respectively.

关 键 词：红曲菌 莫纳可林K 筛选 分类鉴定 

分 类 号：Q93[生物学—微生物学]