辣椒素对舌鳞癌细胞增殖和凋亡影响的初步研究  

Preliminary Study of the Effects of Capsaicin on Human Tongue Squamous Carcinoma cells Proliferation and Apoptosis

作　　者：李风舟[1] 李明 蒋李懿[1] 孟云霞[1] 陈华[1] 

机构地区：[1]广东省口腔医院南方医科大学附属口腔医院,广东广州510280 [2]长沙市口腔医院,湖南长沙410005

出　　处：《现代生物医学进展》2015年第22期4221-4224,共4页Progress in Modern Biomedicine

基　　金：湖南省科技厅国际合作计划基金项目(2013FJ6009);长沙市科技计划基金项目(K1403054-31);广东省医学科研基金项目(A2014115);长沙市科技局课题(K1205021-31)

摘　　要：目的:研究辣椒素对人舌鳞癌细胞增殖和存活的影响。方法:不同浓度辣椒素处理TSCCA和Tca8113细胞24,48和72h,MTS法检测细胞增殖,Western Blot法检测细胞周期调控和细胞凋亡相关分子的表达。结果:随着辣椒素浓度的增加,p53,p21和p27表达上调,Bcl-2表达未受到明显影响,Bcl-XL和Mcl-1表达抑制,caspase7和PARP剪切体表达上调。结论:辣椒素呈剂量依赖和时间依赖性抑制TSCCA和Tca8113细胞增殖,其机制可能与细胞周期和细胞凋亡相关分子表达的改变相关。Objective： To investigate the effects of capsaicin on proliferation and survival of human tongue squamous carcinoma ceils. Methods： TSCCA and Tca8113 ceils were incubated with different concentrations of capsaicin for 24, 48 and 72h, cell proliferation was tested by MTS and western blot was used to detect the expression the molecules which regulated cell cycle and apoptosis. Results： With the increase of the concentration of capsaicin, the expression of p53, p21 and p27 was gradually increased, but bcl-2 failed to be significantly upregulated. The expression of Bcl-XL and Mcl-lwas inhibited, and the cleaved caspase7 and PARP was significantly up-regulated. Conclusion： Capsaicin inhibits the proliferation of TSCCA and Tca8113 cells in a time- and dose-dependent manner. The potential mechanism probably related to the changed expression of the molecules which involved in cell cycle and apoptosis regulation.

关 键 词：舌鳞癌 辣椒素 细胞增殖 细胞凋亡 

分 类 号：R739.8[医药卫生—肿瘤]