Cloning and Characterization of Phytochrome A Gene Fa PHYA from Tall Fescue  

高羊茅光敏色素A基因FaPHYA的克隆与分析（英文）

作　　者：王小利 吴佳海 舒健虹 蔡一鸣 刘晓霞 李小冬 

机构地区：[1]贵州省草业研究所,贵州贵阳550006

出　　处：《Agricultural Science & Technology》2015年第9期1851-1856,共6页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(31360576)~~

摘　　要：The sequence fragment of PHYA, obtained from transcriptome sequencing,was used as the template, and the full-length c DNA sequence of PHYA gene in tall fescue was amplified using 3＇RACE and 5＇RACE techniques. The c DNA sequence of PHYA gene has a complete open reading frame（ORF, 293-6 682 bp）, and it encodes a protein composed of 1 129 amino acids. The N-terminal of Fa PHYA is composed of GAF and Phytochrome domains, and its C-terminal contains two repeated PAS domains, one histidine kinase A domain and one histidine kinase-like ATPase domain. The homology analysis showed that the amino acid sequences of Fa PHYA of tall fescue and PHYAs of gramineous plants have higher homologies（85%）, indicating close genetic relationships. However, the homologies between FaPHYA of tall fescue and PHYAs of monocotyledons are lower, indicating far genetic relationships.以高羊茅转录组学测序获得的PHYA序列为模板,同时结合3’RACE和5’RACE方法从高羊茅中扩增出PHYA基因的全长c DNA序列,命名为Fa PHYA。该序列c DNA全长3 983 bp,具有完整的开放阅读框（ORF,293~3 682 bp）,编码蛋白为1 129个氨基酸,Fa PHYA的N端由GAF和Phytochrome结构域组成;C端包括2个重复的PAS结构域,1个组氨酸激酶A结构域,1个类似组氨酸激酶的ATP酶结构域。对编码蛋白氨基酸序列的同源性分析表明,Fa PHYA与单子叶植物PHYA蛋白的氨基酸序列一致性较高,同源一致性水平达85%以上,亲缘关系较近。与双子叶植物的PHYA蛋白的氨基酸序列一致性较低,亲缘关系相对较远。

关 键 词：Tall fescue Phytochrome A gene CLONING BIOINFORMATICS 

分 类 号：S688.4[农业科学—观赏园艺] Q943.2[农业科学—园艺学]