松树皮多聚原花青素片段化产物的分离鉴定及活性  

Identification and Activities of the Fragmented Product of Polymeric Proanthocyanidins from Pine Barks

作　　者：李娜[1] 但汉龙[1] 姜永新[1] 李美娟[2] 张加研[2] 赵平[1] 

机构地区：[1]西南林业大学云南省木材胶黏剂及胶合制品重点实验室,云南昆明650224 [2]西南林业大学材料工程学院,云南昆明650224

出　　处：《西部林业科学》2015年第5期76-80,共5页Journal of West China Forestry Science

基　　金：国家自然科学基金地区科学项目(31260163);云南省教育厅科学研究基金重大专项(ZD2010006)

摘　　要：采用多种柱层析手段,分别从基于茶多酚的思茅松和马尾松树皮多聚原花青素片段化反应产物中分离得到1个主要产物。通过MS、1H NMR和13C NMR波谱解析,其化学结构鉴定为(-)-表儿茶素-(4β-8)-(-)-表没食子儿茶素3-O-没食子酸酯(1)。结果表明,茶多酚中的(-)-表没食子儿茶素3-O-没食子酸酯(EGCG)在片段化反应中扮演着重要角色,化合物1是EGCG通过4β-8与(-)-表儿茶素C-4位上的正离子键合形成而来。采用DPPH、ABTS自由基清除活性测定方法评价了化合物1的抗氧化活性,其清除DPPH、ABTS自由基的能力均高于茶多酚、多聚原花青素及其片段化总产物,SC50值分别为6.12±0.03 g/m L和41.41±0.66 g/m L。A principal product in the fragmented reaction of polymeric proanthocyanidins from the barks of Pinuskesiya var. langbianensis and P. massoniana with tea polyphenols was isolated by column chromatographies, respec-tively. On the basis of MS,1H NMR and 13C NMR spectroscopic analyses, its structure was established as （-） -epicatechin- （413-8） - （-） -epigallocatechin 3-O-gallate （1）. The results showed that the EGCG in tea polyphe-nols plays an important role in the fragmentation reaction, which attached to the cation at C4 of （-） -epicatechinby 4[3-8 linkness to form 1. The antioxidant activities of i were evaluated by DPPH and ABTS radical scavenging ac-tivity assays. Its scavenging capacities against DPPH and ABTS free radicals with SC50 values of 6. 12±0. 03 g/mLand 41.41±0. 66 g/mL were higher than those of tea polyphenols, polymeric proanthocyanidins and their fragmen-ted products, respectively.

关 键 词：松树皮 思茅松 马尾松 多聚原花青素 片段化产物 分离鉴定 抗氧化活性 

分 类 号：TQ35[化学工程]