1株产α-环糊精葡萄糖基转移酶菌株的产酶条件优化  

Optimized fermentation of a cyclomaltodextrin glucanotransferase producing strain

作　　者：张晓磊[1,2] 郝建华[1] 郑鸿飞[1] 刘均忠[1] 孙谧[1] 

机构地区：[1]农业部海洋渔业可持续发展重点实验室青岛市海洋酶工程重点实验室中国水产科学研究院黄海水产研究所,山东青岛266071 [2]上海海洋大学食品学院,上海201306

出　　处：《中国海洋药物》2015年第5期63-70,共8页Chinese Journal of Marine Drugs

基　　金：国家"十二五"国家高科技研究发展计划项目(2014AA093516)资助

摘　　要：目的提高菌株Y112产α-环糊精葡萄糖基转移酶(α-CGTase)的能力。方法采用单因素法和响应面法,对菌株Y112产酶的培养基成分和培养条件进行了优化。结果最终确定了菌株Y112产酶最佳培养基成分为:玉米淀粉7.5g/L,蛋白胨17.8g/L,酵母浸粉9.1g/L,Mn2+0.6mmol/L,NaCl 50g/L,Na2CO315g·L-1(pH 9.6);最佳产酶的培养条件为:27℃下发酵50h。结论优化后的酶活为优化前的2.8倍。Objective To increase the yield of cyclomaltodextrin glucanotransferase （CGTase） produced by strain Yl12. Methods The fermentation conditions and medium of strain Yl12 were studied by using single factor experiment and the response surface design. Results The optimized liquid medium for the production of α-CGTase was composed of corn starch 7.5 g/L, peptone 17.8 g/L, yeast extract 9. 1 g/ L, Mn2＋ 0. 6 mmol/L, NaCI 50 g/L, Na2COa 1S g/L（pH 9.6）. Incubation at 27℃ for 50 h resulted a high level of α-CGTase production. Conclusion The α-CGTase production was 2.8 times as high as the original conditions.

关 键 词：海洋芽孢杆菌 Α-环糊精葡萄糖基转移酶 产酶条件优化 响应面 

分 类 号：R931[医药卫生—生药学]