河北省特种桑叶中游离氨基酸的含量测定  

Determination of contents of free amino acids in special melberry leaves of Hebei province

作　　者：李松涛[1] 甄占萱[2] 尹志峰[1] 杨鹤松[1] 邓淑华[1] 王良友[1] 

机构地区：[1]承德医学院,河北省中药研究与开发重点实验室,承德067000 [2]承德医学院,河北省高校特产蚕桑技术研发中心,承德067000

出　　处：《食品科技》2015年第9期309-311,315,共4页Food Science and Technology

基　　金：河北省海外高层次人才“百人计划”项目(E2012100002)

摘　　要：测定河北省特种桑叶中游离氨基酸的含量。超声辅助提取桑叶中的游离氨基酸,以异硫氰酸苯酯为柱前衍生化试剂,采用反相高效液相色谱法梯度洗脱测定。液相条件为Kromasil C18色谱柱（4.6 mm×250 mm,5μm）,柱温40℃,流速1 m L/min,检测波长254 nm。16种氨基酸在0.03125~1μmol/m L浓度范围内呈现良好的线性关系,r均大于0.9961,平均回收率为92.2%~101.4%（n=6）,RSD〈5.0%。此方法准确可靠,可用于桑叶及其他药食两用植物中游离氨基酸的含量测定。The free amino acids in special mulberry leaves of Hebei province were ultrasonically extracted with water and their contents were determined by high performance liquid chromatography（HPLC） with phenyl isothiocyanate as pre-column derivatization reagent. The HPLC analysis was performed on a Kromasil C18 chromatographic column（4.6 mm×250 mm, 5 μm） by gradient elution with the flow rate of 1 m L/min at 40 ℃, detected with Ultra violet at 254 nm. In the concentration range of 0.03125~1 μmol/m L,the peak area and concentration of 16 kinds of amino acids showed a good linear relationship,the coefficients of correlation were all over 0.9961, the average recoveries were 92.2%~101.4%（n=6） and the RSD values were all less than 5.0%. The method established was accurate and reliable, which could be available for the determination of free amino acids in mulberry leaves and other medicinal or edible plants.

关 键 词：桑叶 游离氨基酸 柱前衍生 高效液相色谱法 

分 类 号：TS201.4[轻工技术与工程—食品科学]