海马等8种海洋中药体外抗氧化活性的比较  

Comparison of Eight Kinds of Marine Drugs in vitro Antioxidant Activity such as Hippocampus

作　　者：林芳花[1,2] 郑爱娥 李燕秋 朱华[3] 

机构地区：[1]惠州学院生命科学系,广东惠州516007 [2]北京化工大学生命科学与技术学院,北京100029 [3]广西民族大学海洋与生物技术学院,广西南宁530006

出　　处：《安徽农业科学》2015年第29期73-74,共2页Journal of Anhui Agricultural Sciences

基　　金：广东省自然科学基金项目(S2012010010288);广东省科技计划项目(2011B031700062);惠州学院项目(C2110405)

摘　　要：[目的]比较研究海马等8种海洋中药体外抗氧化活性。[方法]测定高锰酸钾还原所需的氧化时间;采用光照核黄素产生超氧阴离子,并测定清除率;采用普鲁士蓝法测定总还原力。[结果]氧化时间从短到长顺序:海马<海龙<海藻<龟甲胶<海螵蛸<龟甲<牡蛎<瓦楞子,其中海马氧化时间21.2 s,与Vc相比无显著差异(P>0.05)。超氧阴离子清除率大小顺序:海螵蛸>龟甲胶>海龙>海马>海藻>龟甲>牡蛎>瓦楞子,其中海螵蛸清除率42.21%,与Vc相比无显著差异(P>0.05)。总还原力强弱顺序:龟甲胶>海龙>海马>海藻>牡蛎>海螵蛸>龟甲>瓦楞子。[结论]海马、海龙、海螵蛸、海藻、龟甲和龟甲胶的抗氧化活性较高,但效果均不及Vc,瓦楞子、牡蛎抗氧化活性较低,龟甲胶抗氧化活性高于龟甲。[ Objective ] Comparative study on antioxidant activity of eight kinds of extraction from marine drugs such as Hippocampus. [ Meth- od ] Measure the oxidation time of reducing the potassium permanganate by the sample; adopt the Riboflavin illumination method to generate superoxide anion and determine the clearance rate; adopt Prussian blue to determine the total reducing power. [ Result] Oxidation time ranged from short to long ： Hippocampus 〈 Syngnathussp 〈 Sargassum 〈 Tortoise-shell glue 〈 Cuttlebone 〈 Tortoise beetle 〈 Oysters 〈 Concha arcae, and the oxidized time of Hippocampus was 21.2 s, which had no significant difference compared to Vc （P 〉 0.05 ）. The superoxide anion clearance rate sorted in order of size was： Cuttlebone 〉 Tortoise-shell glue 〉 Syngnathussp 〉 Hippocampus 〉 Sargassum 〉 Tortoise beetle 〉 Oysters 〉 Concha arcae, the clearance rate of Cuttlebone was 42.21% , which had no significant difference compared to Vc （P 〉 0.05 ）. The reducing power ranged from strong to weak as following ： Tortoise-shell glue 〉 Syngnathussp 〉 hippocampus 〉 Sargassum 〉 Oysters 〉 Cuttlebone 〉 Tortoise beetle 〉 Concha arcae . [ Conclusion] Hippocampus, Syngnathussp, Cuttlebone, Sargassum, Tortoise beetle and Tortoise-shell glue showed a higher antioxidant capacity, but the overall effect of them was not as good as Vc , the antioxidant capacity of Concha arcae and Oys- ters were weak, and the antioxidant capacity of Tortoise-shell glue was better than Tortoise beetle.

关 键 词：海马 海洋药物 抗氧化活性 

分 类 号：S986.2[农业科学—捕捞与储运]