柱前衍生高效液相色谱法测定糜子发芽过程中氨基酸变化  

Determination of Amino Acids by High Performance Liquid Chromatography with Pre-column Derivatization during Proso Millet Germination

作　　者：冀佩双 单璐[1] 吕国涛[1] 牛宇[2] 乔治军[3] 张丽珍[1] 

机构地区：[1]山西大学生命科学学院,山西太原030006 [2]山西省农业科学院农业资源与经济研究所,山西太原030006 [3]山西省农业科学院农作物品种资源研究所农业部黄土高原作物基因资源与种质创制重点实验室,山西太原030031

出　　处：《山西农业科学》2015年第10期1230-1233,1236,共5页Journal of Shanxi Agricultural Sciences

基　　金：国家现代农业(谷子糜子)产业技术体系建设专项(CARS-07-12.5-A12);"十二五"国家科技支撑计划项目(2014BAD07B03-01)

摘　　要：采用柱前衍生高效液相色谱法测定了糜子发芽过程中氨基酸的变化规律。以6-氨基喹啉基-N-羟基琥珀酰亚氨基甲酸酯(AQC)为衍生试剂,以磷酸盐缓冲液为流动相A,乙腈/水(6∶4)为流动相B进行梯度洗脱;在柱温37℃条件下用紫外检测器在波长248 nm下进行测定。结果表明,糜子中谷氨酸(Glu)含量最高,其次是亮氨酸(Leu)、丙氨酸(Ala);发芽时间为48 h时,总氨基酸含量和必需氨基酸含量达到了最大;在发芽过程中氨基酸含量增加30%以上的有甘氨酸(Gly)、组氨酸(His)、苏氨酸(Thr)、酪氨酸(Tyr),其中,组氨酸(His)的增幅最大,为51.36%;天冬氨酸(Asp)、丝氨酸(Ser)、精氨酸(Arg)、丙氨酸(Ala)在发芽过程中含量变化不显著。The variation of the amino acid contents was determined by high performance liquid chromatography with pre-column derivatization during proso millet germination. Amino acids were derivatized by 6-aminoquinolyl-N-Hydroxysuccinimidyl-carbamate（AQC）, and then their derivatives were separated with a column temperature of 37 ℃ using phosphate buffer as mobile phase A and aqueous acetonitrile as mobile phase B in the gradient elution mode and detected at 248 nm using a UV detector. The results showed that the content of Glu was the highest in the detected amino acids of the proso millet, followed by Leu, Ala. The contents of total amino acids and essential amino acids reached the maximum when the germination time was 48 h. The contents of glycine（Gly）, histidine（His）,threonine（Thr）, tyrosine（Tyr）increased by over 30%, and the histidine（His）increased by 51.36% which was the largest. The contents of aspartic acid（Asp）, serine（Ser）, arginine（Arg）, alanine（Ala）did not change significantly.

关 键 词：柱前衍生 高效液相色谱法 糜子 氨基酸 

分 类 号：S516[农业科学—作物学]