基于基因芯片的前列腺癌转移高表达VCAN基因的生物信息学分析  

Bioinformatics analysis of prostate cancer metastasis with highly expressed gene VCAN based on microarray

作　　者：李铁求[1] 滕伊漓[1] 邹亚光[2] 毛向明[3] 

机构地区：[1]湖南省马王堆医院泌尿外科,长沙410016 [2]南方医科大学附属南方医院口腔科 [3]北京大学深圳医院泌尿外科

出　　处：《中国医师杂志》2015年第9期1364-1368,共5页Journal of Chinese Physician

摘　　要：目的 利用生物信息学方法分析前列腺癌转移相关VCAN基因以及蛋白的结构,为进一步研究其功能和参与的调控机制提供一定的理论依据.方法 在公共基因芯片数据库（GEO）中下载前列腺癌转移的相关基因芯片数据,利用BRB-ArrayTools软件、protparam、SMART、SignalP 4.0、TMHMM、NetPhos2.0、PredictProtein、SWISS-MODEL、GO、KEGG、STRING等生物信息学工具进行数据挖掘及生物信息学分析.结果 共筛选出前列腺癌转移共同差异基因73个,其中表达上调21个,表达下调52个,其中对前列腺癌转移高表达基因VCAN进行生物信息学发现,VCAN蛋白由3 396个氨基酸组成,该蛋白含有1个IG结构域、2个Link结构域,1个EGF结构域、1个EGF_CA结构域、1个CLECT结构域和1个CCP结构域,主要参与细胞黏附、透明质酸结合、钙离子结合、糖胺聚糖结合、细胞外基质、细胞黏附分子等分子功能及信号通路.结论 利用生物信息学的方法能有效分析基因芯片数据并获取生物内在信息,VCAN可能在前列腺癌转移中发挥重要作用,有望成为前列腺癌转移的早期诊断标志物和治疗的新靶点.Objective To investigate the function and regulatory mechanisms of VCAN gene and protein in metastatic prostate cancer.Methods The data of whole genomic expression profiles got from the prostate cancer metastasis were obtained from gene expression omnibus （GEO） database,a set of bioinformatics tools,such as BRB-Array Tools,protparam,SMART,SignalP 4.0,TMHMM,NetPhos2.0,PredictProtein,SWISS-MODEL,GO,KEGG and STRING softwares were used to accomplish data-mining and bioinformatics analysis.Results There were 73 co-expressed differentially genes in prostate cancer metastasis,21 up-regulated and 52 down-regulated.Bioinformatics analysis indicated that VCAN gene encoded 3396 amino acids,VCAN was also contained one Immunoglobulin domain,two hyaluronan-binding domain,one epidermal growth factor-like domain,one calcium-binding EGF-like domain,one C-type lectin domain and one domain abundant in complement control proteins,and a furthermore analysis suggested that VCAN played essential roles in such important biological function including cell adhesion,hyaluronic acid binding,calcium-binding,glycosaminoglycan binding,extracellular matrix and cell adhesion molecules.Conclusions Bioinformatics analysis had a high efficiency in analyzing microarray data and revealing internal biology information.VCAN may play an important role in the prostate cancer metastasis,Thus,VCAN might be a novel biomarker for the diagnosis of prostate cancer metastasis or a new target for its treatment.

关 键 词：寡核苷酸序列分析 计算生物学 前列腺肿瘤/遗传学 肿瘤转移/遗传学 基因 

分 类 号：R737.25[医药卫生—肿瘤]