活性维生素D3对IgA肾病大鼠肾组织中Toll样受体4表达的影响  

Effects of 1, 25- dihydroxyvitamin D3 on renal expression of TLR-4 in Ig AN rats

作　　者：王缨[1] 汪国平[1] 李弼民[1] 

机构地区：[1]南昌大学第一附属医院肾内科,江西南昌330006

出　　处：《中国现代医学杂志》2015年第25期25-29,共5页China Journal of Modern Medicine

摘　　要：目的探讨活性维生素D3对Ig A肾病(Ig AN)大鼠保护作用的可能机制。方法 50只大鼠随机分为3组,正常对照组(NC组)10只、对照模型组(MC组)20只、活性维生素D3治疗组(干预组)20只。干预组给予活性维生素D3灌胃8周。灌胃第4和8周末检测24 h尿蛋白定量、肾功能及血浆清蛋白,采用酶联免疫吸附测定血浆白细胞介素-1(IL-1)蛋白表达,免疫组织化学法检测肾组织Toll样受体4(TLR4)蛋白的表达。结果 1与MC组比较,干预组血肌酐和24 h尿蛋白均下降(均P<0.05);2与NC组比较,MC组TLR4蛋白表达增加(P<0.05);与MC组比较,干预组TLR4蛋白表达下降(P<0.05);3与NC组比较,MC组IL-1表达增加(P<0.05);与MC组比较,干预组IL-1表达下降(P<0.05)。结论活性维生素D3可通过抑制Ig AN大鼠的TLR4的表达及细胞因子IL-1的表达,对Ig AN大鼠肾脏损伤有预防保护作用。【Objective】To explore possible renal protective effect of 1, 25-dihydroxyvitamin D3 on Ig AN rats.【Methods】Fifty rats were randomized into 3 groups, the normal control group（group NC）（n = 10）, Ig AN group（group MC）（n = 20）, Ig AN rats treated with 1, 25- dihydroxyvitamin D3（Intervention group）（n = 20）. After 4 and 8weeks treatment, 24 h urine protein, serum creatinine and serum albumin were detected. ELISA was used to monitor the expressions of interleukin-1（IL-1） protein in plasma. Immunohistochemical staining was used to monitor the expressions of Toll-like receptor 4（TLR4） protein in renal tissue.【Results】124 h urine protein and serum creatinine in the intervention group were significantly lower than those in the MC group（P〈0.05）; 2TLR4 protein expression in MC group was significantly higher than that in NC group（P〈0.05）, and the expression in the intervention group was significantly lower than that in MC group（P〈0.05）; 3IL-1 expression in the MC group was significantly higher than that in NC group（P〈0.05）; IL-1 expression in the intervention group was significantly lower than that in MC group（P〈0.05）. 【Conclusion】1, 25- dihydroxyvitamin D3 has some renal protective effect on Ig AN rats,partly by down- regulating TLR4 in renal tissue.

关 键 词：IG A肾病 活性维生素D3 TOLL样受体4 白细胞介素-1 大鼠 

分 类 号：R-332[医药卫生] R692