Yarrowia lipolytica酵母产赤藓糖醇的工艺研究  

Research on the process for erythritol production by Yarrowia lipolytica yeast

作　　者：田强 秦海青 邱学良 梁学超 刘海朋 袁敏 

机构地区：[1]山东福田药业有限公司,禹城251200

出　　处：《中国食品添加剂》2015年第9期121-124,共4页China Food Additives

摘　　要：对自行筛选诱变得到的高产菌（Yarrowia lipolytica）FT-3进行发酵条件优化试验。最佳培养基组成为：葡萄糖32%,酵母粉0.6%,柠檬酸铵0.5%,硫酸镁0.025%,p H自然;移种的最佳条件为：培养时长20h,出芽率50%,细胞密度8×108~10×108/m L;最佳发酵条件,温度：前期28℃,对数期和发酵中期30℃,发酵后期32℃;溶氧30%~40%,通气量7NL/min,约1.0vvm。在此条件下,发酵结束后醪液中赤藓糖醇的浓度达到187g/L。In this paper,the optimization of the fermentation conditions of FT-3（Yarrowia lipolytica）was carried out. The best culture medium was glucose 30%,yeast powder 0.6%,citric acid ammonium0.5%,magnesium sulfate 0.025%,natural p H. The best inoculation conditions were ：culture time 20 h,the germination rate 50%,the cell density 8×108 ~ 10×108/m L. Optimal fermentation were ：temperatures – early stage 28 ℃,mid log phase 30 ℃,fermentation period 32℃;dissolved oxygen content 30% ~ 40%,ventilation 7NL/min,about 1.0vvm. Under these conditions,the concentration of erythritol in final fermented liquid could reach to 87g/L.

关 键 词：赤藓糖醇 YARROWIA lipolytica 发酵优化 

分 类 号：TS202.3[轻工技术与工程—食品科学]