HPLC-CAD法测定乳制品中果糖、葡萄糖、蔗糖、乳糖、半乳糖和麦芽糖含量的不确定度评定  

Determination of fructose,glucose,sucrose,lactose,galactose and maltose in dairy products by HPLC-CAD method

作　　者：杨勇[1,2] 吴琳琳[3] 罗奕[3] 游正琴 史蕙[2] 许乾丽[2] 杨园园[2] 

机构地区：[1]贵阳中医学院,贵阳550002 [2]贵州省食品药品检验所,贵阳550004 [3]贵阳医学院,贵阳550004

出　　处：《中国食品添加剂》2015年第9期172-180,共9页China Food Additives

基　　金：食品药品非法添加化工染料的快速检测及相关数据库的研究(黔科合SY字[2011]3090号);贵州省食品;保健食品和化妆品监测检测平台(黔科平台[2012]4001);餐饮服务食品色素检测研究.国家食品药品监督管理局研究课题

摘　　要：目的采用高效液相色谱(HPLC)串联电喷雾检测器(CAD)法同时测定乳制品中果糖、葡萄糖、蔗糖、乳糖、半乳糖和麦芽糖的含量,分析果糖、葡萄糖、蔗糖、乳糖、半乳糖和麦芽糖不确定度。方法通过HPLC-CAD法测定乳制品中果糖、葡萄糖、蔗糖、乳糖、半乳糖和麦芽糖的含量方法建立数学模型,对各个不确定度因素进行评估、计算,由此计算合成不确定度,最终给出测量结果在95%置信区间下的扩展不确定度。结果本次实验含量测定结果为果糖2.15±0.009(g/100g)葡萄糖7.7490±0.010(g/100g)蔗糖24.5571±0.014(g/100g)乳糖19.8333±0.021(g/100g)半乳糖1.0168±0.026(g/100g)麦芽糖1.8727±0.019(g/100g)。结论所建方法可行,适用于乳制品中果糖、葡萄糖、蔗糖、乳糖、半乳糖和麦芽糖含量的测定。objective ：using high performance liquid chromatography（HPLC）and tandem electrospray ionization detector（CAD）method to determinate the content of dairy products in fructose,glucose,sucrose,lactose and maltose simultaneously,analyzing the uncertainty of fructose,glucose,sucrose,lactose and maltose. Method ：using the developed hplc-cad method to determinate the content of dairy products in fructose,glucose,sucrose,lactose and maltose to establish mathematical model,of various uncertain factors are evaluated andcalculated thus calculated synthetic uncertainty,finally gives the measurement results at 95% confidence intervals for the expansion uncertainty. Results ：the experimental content determination results are fructose 2.15 0.009（g/100g）glucose 7.7490 0.010（g/100g）sucrose 24.5571 0.014（g/100g）lactose 19.8333 0.021（g/100g）Galactose 0.026（g/100g）malt sugar 1.8727 0.019（g/100g）. Conclusion ：the method is feasible and suitable for the determination of fructose,glucose,sucrose,lactose and maltose in dairy products..

关 键 词：乳制品 高效液相色谱-电喷雾检测器法 不确定度 评定 

分 类 号：TS207.3[轻工技术与工程—食品科学]