7型猪链球菌免疫相关性蛋白乳酸脱氢酶的筛选与分析  

Screening and analysis of immune related proteins of Streptococcus suis serotype 7

作　　者：高明明[1] 王淑杰[1] 刘永刚[1] 姜成刚[1] 王刚[1] 涂亚斌[1] 蔡雪辉[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001

出　　处：《西北农林科技大学学报（自然科学版）》2015年第10期1-6,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家科技重大专项(2012ZX10004214);国家自然科学基金项目(31060281)

摘　　要：【目的】快速高效筛选7型猪链球菌免疫原性蛋白,以作为猪链球菌疫苗的候选分子。【方法】以7型猪链球菌分离株WH07为样品,应用免疫蛋白质组学技术建立了WH07株全菌蛋白的双向电泳体系,并利用蛋白质免疫杂交技术筛选免疫原性蛋白。【结果】获得了分辨率及重复性良好的双向电泳（2-DE）图谱,且得到3个具有免疫反应性的蛋白质点,经质谱鉴定其归属于1个蛋白,即乳酸脱氢酶（LDH）。生物信息学预测表明,该蛋白分子质量为35 420ku,理论等电点为5.05,具有2个跨膜区,分别位于第10~28位和第110~129位,并根据预测分析结果成功模拟了蛋白质的三级结构。【结论】从猪链球菌WH07菌株中筛选并鉴定了1个具有免疫原性的蛋白,即乳酸脱氢酶,并模拟了其三级结构。【Objective】This study aimed to identify the immune-associated proteins of Streptococcus suis serotype 7as S.suis vaccine candidates.【Method】The two-dimensional gel electrophoresis（2-DE）for S.suis proteome separation was established for S.suis WH07 strain and the immune-related proteins were identified by western blot.【Result】Three proteins were recognized by S.suis positive serum and identified as L-lactate dehydrogenase（LDH）by mass spectrometry analysis.The molecular mass of the predicted protein was 35 420 ku,its pI was 5.05,and there were two trans-membrane regions at 10-28 and 110-129,respectively.The tertiary structure of the protein was also successfully simulated according to the forecast analysis.【Conclusion】This study screened and identified one immune-related protein from strain WH07 and simulated its tertiary structure.

关 键 词：猪链球菌 免疫相关蛋白 双向电泳 质谱鉴定 蛋白结构预测 

分 类 号：S852.611[农业科学—基础兽医学]