滇重楼茎叶总皂苷提取工艺优化及其体外抗氧化活性分析  

Extraction and Antioxidant Activity of Total Saponins from Stems and Leaves of Paris polyphylla var. yunnanensis( Franch. ) Hand.-Mazz.

作　　者：韦蒙 许新恒 李俊龙[1] 申世安[1] 刘静[1] 丁春邦[1] 

机构地区：[1]四川农业大学生命科学学院,雅安625014

出　　处：《天然产物研究与开发》2015年第10期1794-1800,共7页Natural Product Research and Development

基　　金：国家大学生创新实验计划(201410626009)

摘　　要：以滇重楼茎叶为试验材料,采用响应面法优化超声波辅助提取滇重楼茎叶总皂苷的工艺,并分析总皂苷的体外抗氧化活性。在单因素试验的基础上,利用Box-Benhnken设计四因素五水平响应面试验,确定最佳提取工艺条件为料液比1∶12(g/m L)、提取温度54℃、超声功率210 W、提取时间2 h,滇重楼茎叶总皂苷实际平均得率为2.360%,预测得率为2.385%,相对误差为1.04%。滇重楼茎叶总皂苷对DPPH自由基、羟自由基和超氧阴离子的最大清除率分别为98%、58%和64%,其IC50分别为2.223、6.782 mg/m L和4.638 mg/m L。Response surface methodology （RSM） was used to optimize the ultrasonic-assisted e：itraction process of total saponins from stetr/s and leaves of Pairs polyphylla var. yunnanensis （ Franeh. ） Hand. -Mazz. Furthermore, the antioxidant activity of the total saponins was analyzed in vitro. Based on single-factor tests, a five-level four-factor response surface test was developed by Box-Benhnken design. The optimal extraction conditions were determined to be the solid-liquid ratio of 1：12 （g/mL）, extraction temperature of 54 ℃, ultrasonic power of 210 W, and extraction time of 2 h. Under these conditions,the extraction yield was 2. 360% ,while the predicted extraction yield was 2.385% ,with relative error of 1.04%. The maximum inhibition rates of DPPH, hydroxyl radical and superoxide anion of the total saponins were 98% ,58% and 64% ,respectively. The IC50 values of those antioxidant activities were 2. 223 mg/mL,6.782 mg/mL and 4.638 mg/mL,respectively.

关 键 词：滇重楼茎叶 总皂苷 响应面法 超声波辅助提取 抗氧化 

分 类 号：TS201.1[轻工技术与工程—食品科学]