EGCG联合姜黄素对人卵巢癌细胞增殖的抑制作用  

Inhibitory effect of EGCG combined with curcumin on proliferation of human ovarian cancer cells

作　　者：吴溧兴[1] 许小进[1] 陈晓栋[1] 王学敏[2] 傅士龙 周冬虎[2] 李媛[2] 冯晴[2] 

机构地区：[1]南京中医药大学附属中西医结合医院心血管病科,江苏省210028 [2]南京医科大学公共卫生学院营养与食品卫生学系 [3]南京医科大学第一附属医院河西分院妇科

出　　处：《江苏医药》2015年第20期2367-2370,F0002,共5页Jiangsu Medical Journal

基　　金：国家自然科学基金(81472977);教育部留学回国人员启动基金(HG11-4302);江苏省"六大人才高峰"资助项目;江苏省高校优势学科建设工程资助项目

摘　　要：目的研究表没食子儿茶素没食子酸酯(EGCG)联合姜黄素对人卵巢癌细胞株A2780增殖的抑制作用及其可能机制。方法将A2780细胞随机分为空白对照组(A组)、EGCG 10μmol/L处理组(B组)、姜黄素5μmol/L处理组(C组)和EGCG 10μmol/L+姜黄素5μmol/L联合处理组(D组)。采用MTT法检测A2780细胞存活率,5-乙炔基-2′-脱氧尿苷(EdU)荧光染色检测细胞DNA复制活性,平板集落形成实验观察单个细胞的生存能力,Western blot法检测细胞周期蛋白B1(Cyclin B1)和Cyclin D1的表达。结果 EGCG和姜黄素均呈剂量依赖性地抑制A2780细胞增殖(P<0.05)。与A、B、C组相比,D组细胞存活率、EdU染色阳性细胞数、细胞集落形成率、Cyclin B1和Cyclin D1表达均降低(P<0.05或P<0.01)。结论 EGCG联合姜黄素能明显抑制A2780细胞增殖;其机制可能与诱导细胞周期阻滞有关。Objective To investigate the inhibitory effect and underlying mechanism of epigallocatechin gallate（EGCG）combined with curcumin on the proliferation of human ovarian cancer cell line A2780.Methods A2780 cells were randomly divided into four groups of A（blank control）,B（treated with EGCG 10μmol/L）,C（treated with curcumin 5μmol/L）and D（treated with EGCG10μmol/L plus curcumin 5μmol/L）.The cell survival was measured by MTT assay,and DNA replication in A2780 cells was determined by EdU fluorescence staining.The single cell viability was observed by colony formation assay.The expressions of Cyclin B1 and Cyclin D1 were detected by Western blot.Results Both EGCG and curcumin could inhibit cell proliferation in a dose-dependent manner（P〈0.05）.Compared with groups of A,B and C,the cell survival,positive cells of EdU staining,rate of cell colony formation and expressions of Cyclin B1 and Cyclin D1 were significantly decreased in group D（P〈0.05 or P〈0.01）.Conclusion EGCG combined with curcumin can remarkably inhibit the proliferation of A2780 cells,which may be related with cell cycle arrest.

关 键 词：卵巢癌 姜黄素 表没食子儿茶素没食子酸酯 

分 类 号：R737[医药卫生—肿瘤]