Identification of antidepression constituents from Angelica Sinensis Radix contrubuting to Xiaoyaosan  

出　　处：《中国药理学通报》2015年第B11期120-121,共2页Chinese Pharmacological Bulletin

基　　金：国家国际科技合作项目（No2011DFA32630）;国家科技部“重大新药创制”科技重大专项（No2012ZX09103201-035）;山西省科技攻关项目（No20140313008-14）;山西省科技基础条件平台建设项目（No2014091022）.

摘　　要：Aim The study focused on identification and antidepression active verification of constituents from An- gelica Sinensis Radix contrubuting to Xiaoyaosan based on UPLC-PDA guided isolation technique. Methods The UPLC-PDA chromatogram of Xiaoyaosan was compared with that of Angelica Sinensis Radix. The relative retention time of each peak and the Uhraviolet spectra providing by PDA were used in the analyses. Constituents were isola- ted from Angelica Sinensis Radix under the guidance of UPLC-PDA investigation. Structures of the isolates were elu- cidated by NMR techniques. Anti-depression effect was evaluated on glutamate-induced neurons. Results Five marker peaks of Xiaoyaosan fingerprint were belong to Angelica Sinensis Radix, and they were determined as conife- ryl ferulate（ I）, E-butylidenephthalide （ II）, ligustilide （III）, Z-butylidenephthalide （ IV ）, 14-Acetoxy-12-sene- cioyloxytetradeca-2E,8E,10E-trien-4,6-diyn-l-ol（V）. Compound V was isolated from the plants of Umbelliferae for the first time. Treatment with compound I, III, IV can protect PC12 and SH-SY5Y cell from glutamate-induced cytotoxicity. Antidepression bioactivity of compound I was first investigated. Conclusion UPLC-PDA guided iso-lation technique was confirmed to be a rapid and accurate method to identify the main active constituents from An- gelica Sinensis Radix contrubuting to Xiaoyaosan.

关 键 词：Xiaoyaosan ANGELICA SINENSIS RADIX GUIDED ISOLATION antidepression UPLC-PDA ACTIVE constitu-ents 

分 类 号：R[医药卫生]