Solasodine induces apoptosis and inhibits metastasis on ovarian cancer HEY cells  

出　　处：《中国药理学通报》2015年第B11期240-240,共1页Chinese Pharmacological Bulletin

摘　　要：Aim Solasodine, a steroidal alkaloid isolated from solanaceous species, has been reported to exhibit anticancer activity on several cell lines. The aim of this study is to explore the antitumor potential of solasodine on ovarian cancer HEY cells. Methods MTT assay, Hoechst 33342 staining, JC-1 mitochondrial membrane potential assay, and LDH release assay were used to study the anti-proliferative and apoptosis-inducing effects of solasodine. MDC staining was to label the acidic puncta in the cells. Meanwhile, wound healing assay and transwell assay were performed to determine whether solasodine exhibits anti-metastasis effect on HEY cells. Gelatin zymography assay was used to determine the activities of MMP-2 and MMP-9 and Western blot was used to examine the expression of the relevant proteins. Results Solasodine inhibited the cell viability on HEY cells in time- and dose-dependent manners, causing shrinking of nuclei, damage of cell membrane, and appealing apoptotic body on high dosage and reducing cell mitochondrial membrane potential. It also inhibited autophagic flux that interfere autolysosome degra- dation. Furthermore, solasodine suppressed the migration and invasion abilities of HEY cells ascribable to down- regulating the expression and attenuating the activities of MMP-2 and MMP-9. Conclusion The inducing apoptosis and anti-metastasis activity of solasodine on HEY cells suggest it appears certain potential on ovarian cancer treat- ment.

关 键 词：OVARIAN cancer SOLASODINE APOPTOSIS autophagy migration INVASION 

分 类 号：R[医药卫生]