醒酒玉米低聚肽的分离制备及其氨基酸序列分析  

Preparation,separation and amino acid sequence analysis of corn oligopeptides with antialcoholism activity

作　　者：马伟[1,2] 戴军[2] 陈尚卫[2] 朱松[2] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江南大学食品学院食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《食品与机械》2015年第5期197-200,221,共5页Food and Machinery

摘　　要：以脱淀粉玉米蛋白粉为原料,用Alcalase 2.4L在优化酶解条件下制得玉米低聚肽产品COP。通过体外醒酒活性及动物试验表明:COP具有较高体内外醒酒活性,同时发现COP的剂量与小鼠血醇含量的降低呈明显的剂量—效应关系。经凝胶过滤色谱和反相色谱分离分级及醒酒活性筛选,得到醒酒活性较强的玉米低聚肽级分COPⅢ-17。该级分通过超高效液相色谱—四级杆—飞行时间串联质谱联用分析,得到其氨基酸序列为:P-Y-L-P-L-L-P-S。Corn oligopeptides（COP）were prepared via enzymatic hydrolysis of destarched corn proteins by Alcalase 2.4L.By investigated COP antialcoholism activity in vitro and in vivo,it is found that COP has highly antialcoholism activity.Meanwhile,the scavenging rate of blood alcohol concentration is positively correlated with the dose of COP.COP were separated by Sephadex G-15 gel chromatogram and RP—HPLC step by step,then fraction COPⅢ-17 was separated with highly antialcoholism activity.UPLC—Q—TOF—MS analysis showed that its amino acid sequence：P-Y-L-P-L-L-P-S.

关 键 词：玉米 低聚肽 醒酒活性 色谱分离 四级杆—飞行时间串联质谱 

分 类 号：TS213.4[轻工技术与工程—粮食、油脂及植物蛋白工程]