15个常染色体和10个Y-STR基因座复合扩增试剂盒的研发  

Development of a Forensic Multiplex Amplification STR Kit for 15 Autosomal STR Loci and 10 Y-STR Loci

作　　者：董研[1,2] 林双双[3] 曹禹[1] 吴微微[4] 黄书琴[5] 郑卫国 李发院 葛斌文 郭育林 周怀谷[1] 

机构地区：[1]上海市公安局物证鉴定中心法医物证学现场应用技术公安部重点实验室上海市现场物证重点实验室,上海200083 [2]复旦大学生命科学学院,上海200433 [3]上海市公安局长宁分局,上海200335 [4]浙江省公安厅刑侦总队,浙江杭州310009 [5]郑州市公安局,河南郑州450000 [6]无锡中德美联生物技术有限公司,江苏无锡214174

出　　处：《法医学杂志》2015年第5期373-376,380,共5页Journal of Forensic Medicine

基　　金：上海市科研计划项目(13JG0500200)

摘　　要：目的建立法医物证学常染色体和Y染色体综合检测的方法。方法选择常用的常染色体STR基因座和Y染色体STR基因座,设计复合扩增引物,形成五色荧光标记复合扩增试剂盒。结果开发出一个五色荧光标记复合扩增试剂盒,可同时对15个常染色体STR基因座、1个性别基因座和10个Y染色体STR基因座进行分型检测。结论 15个常染色体STR加10个Y染色体STR检测试剂盒结合毛细管电泳凝胶进行STR分型,结果准确可靠,在法医学案件检验及数据库建设等方面有良好的应用前景。Objective To establish a multiplex STR genotyping method for autosomal STR and Y-STR loci in forensic biological practice. Methods Widely used autosomal STR loci and Y-STR loci were se- lected. A set of PCR primers was designed, and a 5-dye fluorescent labeled STR multiplex PCR reagent kit was developed. Results A kit was developed which can simultaneously detect 15 autosomal STR loci, 10 Y-STR loci, and an Amelogenin. Conclusion The 15 autosomal STR plus 10 Y-STR kit in combination with capillary eleetrophoresis method was used to STR genotyping with accurate and reli- able results. The new one-step testing kit can potentially be widely used in forensic cases and DNA databank in the future.

关 键 词：法医遗传学 试剂盒 短串联重复序列 常染色体 Y染色体 

分 类 号：R394[医药卫生—医学遗传学]