酶法原位分离技术制备葡萄糖酸  

Preparation of gluconic acid by enzymic process coupled with in situ product removal

作　　者：魏胜华[1,2] 王卫军[1] 王宏[3] 朱必玉 钱伟[1] 孟娜[1,2] 李世文[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000 [2]微生物发酵安徽省工程研究中心,安徽芜湖241000 [3]安徽工程大学机电学院,安徽芜湖241000

出　　处：《精细化工》2015年第11期1217-1221,1235,共6页Fine Chemicals

基　　金：国家级大学生创新创业训练计划基金资助项目(3120402123)~~

摘　　要：研究了使用聚乙烯醇-海藻酸钠固定化的葡萄糖氧化酶-过氧化氢酶作为催化剂,在鼓泡式反应器中采用原位分离技术转化葡萄糖制备葡萄糖酸的过程。研究发现,该转化过程受到产物的抑制作用,通过对树脂的筛选,采用对葡萄糖酸具有较好吸附效果的D335树脂,可以解除产物的抑制作用。结合流加补料的转化方式,葡萄糖酸的转化率可以达到95.5%。连续使用4个批次,其平均转化率在85.7%以上,系统的稳定性较好。The glucose oxidase and catalase immobilized in polyvinyl alcohol and sodium alginate matrix as biocatalyst for conversion of glucose to gluconic acid in bubbling reactor coupled with in situ product removal was investigated. The conversion was inhibited by glucose acid. Using the D335 resin,which has good adsorption capacity of gluconic acid,could relieve the inhibition. By the fed batch-in situ removal,gluconic acid conversion could reach 95. 5%. The system was stable,it could successively convert for four cycles,and the average conversion was more than 85. 7%.

关 键 词：葡萄糖酸 原位分离 吸附 鼓泡式反应器 生物工程 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]