微量量热法研究淀粉酶催化反应  

Microcalorimetric Study on Enzyme Catalyzed Hydrolysis Reaction of Starch

作　　者：于秀芳[1] 张洪林[1] 张刚[2] 

机构地区：[1]曲阜师范大学化学系,曲阜273165 [2]山东大学微生物技术国家重点实验室

出　　处：《应用化学》2002年第8期812-813,共2页Chinese Journal of Applied Chemistry

基　　金：山东省自然科学基金资助项目 (Y2 0 0 0 B0 3)

摘　　要：The thermal power time relation of starch hydrolysis reaction catalyzed by hydrolytic enzyme has been evaluated using a microcalorimeter . The maximum velocity( V max ) was calculated according to the equation V max =[-2 975 79×10 -4 +2 401 91×10 -4 pH-6 940 00×10 -5 pH 2+ 9 040 00× 10 -6 pH 3-4 450 00×10 -7 pH 4] g/(dm 3·s) .The optimum acidity was found to be pH=5 78.The thermal power time relation of starch hydrolysis reaction catalyzed by hydrolytic enzyme has been evaluated using a microcalorimeter . The maximum velocity( V max ) was calculated according to the equation V max =[-2 975 79×10 -4 +2 401 91×10 -4 pH-6 940 00×10 -5 pH 2+ 9 040 00× 10 -6 pH 3-4 450 00×10 -7 pH 4] g/(dm 3·s) .The optimum acidity was found to be pH=5 78.

关 键 词：淀粉酶 淀粉 酶催化水解反应 热动力学 微量量热法 

分 类 号：O636.1[理学—高分子化学] O643.32[理学—化学]