高效液相色谱法同时测定8种丛生竹竹叶中香豆素类成分  

Simultaneous Determination of Coumarins in Leaves of Eight Sympodial Bamboo Species with High Performance Liquid Chromatography

作　　者：姚曦 岳永德 汤锋 

机构地区：[1]国际竹藤中心,北京100102

出　　处：《食品科学》2015年第20期105-108,共4页Food Science

基　　金："十二五"国家科技支撑计划项目(2012BAD23B03)

摘　　要：竹叶用体积分数80%乙醇为提取溶剂进行快速微波辅助萃取,采用高效液相色谱法测定样品中10种香豆素类化学成分（茵芋苷、东莨菪苷、东莨菪内酯、伞形酮、6,7-二甲氧基香豆素、香豆素、补骨脂素、花椒毒素、5,7-二甲氧基香豆素、茴芹内酯）。标准品连续5次进样和在3 d内多次进样,峰面积的相对标准偏差均不高于3.47%。对2属8个丛生竹竹种（梁山慈竹、牡竹、龙竹、勃氏甜龙竹、硬头黄竹、黄金间碧玉竹、粉单竹和青皮竹）竹叶样品分析,共检出5种香豆素类化合物,含量在2.07～67.54 mg/kg之间。黄金间碧竹中总香豆素含量最高,为103.77 mg/kg,梁山慈竹中总香豆素含量最低,为27.32 mg/kg。簕竹属组的4种竹叶香豆素总体含量水平明显高于牡竹属组。Coumarins from the leaves of eight sympodial bamboo species（Dendrocalamus farinosus, Bambusa rigida, Bambusa vulgaris, Bambusa textilis, Bambusa chungii, Dendrocalamus strictu, Dendrocalamus giganteus, and Dendrocalamus brandisii） were extracted with ethanol-water（80：20, V/V） using a microwave-assisted method. A high-performance liquid chromatography（HPLC） method for separating and determining ten coumarin standards including skimin, scopolin, scopoletin, umbelliferone, 6,7-dimethoxycoumarin, coumarin, psoralen, xanthotoxin, 5,7-dimethoxycoumarin, and pimpinellin was developed and used to analyze the coumarins extracted from the leaves of sympodial bamboo. Relative standard deviations（RSD） of peak area were below 3.47% in fi ve consecutive tests and repeated tests during three days. A total of fi ve coumarins were detected at contents ranging from 2.07 to 67.54 mg/kg. The maximum amount of total coumarins was found in Bambusa vulgaris with content of 103.77 mg/kg, while the minimum amount of 27.32 mg/kg was found in Dendrocalamus farinosus. The contents of total coumarins in the leaves of four Bambusa species were obviously higher than in four Dendrocalamus species.

关 键 词：丛生竹 竹叶 香豆素 高效液相色谱 

分 类 号：TS207.3[轻工技术与工程—食品科学]