离子色谱法测定黑果枸杞中的甜菜碱  

Determination of Betaine in Lycium ruthenicum Murr. by Ion Chromatography

作　　者：耿丹丹[1,2] 谭亮[1] 肖远灿[1] 胡风祖[1] 董琦[1] 

机构地区：[1]中国科学院西北高原生物研究所,青海西宁810008 [2]中国科学院大学生命科学学院,北京100049

出　　处：《食品科学》2015年第20期145-147,共3页Food Science

基　　金：中国科学院仪器功能开发技术创新项目(Ig201308)

摘　　要：建立黑果枸杞中甜菜碱的离子色谱测定法。采用Metrosep C4阳离子分析柱（150 mm×4.6 mm,4μm）,柱温为38.5℃,1.5 mmol/L甲烷磺酸溶液为流动相,流速为0.8 m L/min,电导检测器。结果表明,甜菜碱在10.4～830.0 mg/L质量浓度范围内线性关系良好（R2=0.999 8）,加标回收率在95.51%～98.06%（n=3）之间。所建方法操作简便、重复性好,适用于黑果枸杞中甜菜碱含量测定。An ion chromatography coupled with conductivity detection method was developed for the determination of betaine in Lycium ruthenicum Murr.. The analyte was separated on a Metrosep C4 cation exchange column（150 mm ×4.6 mm, 4 μm） by using methane sulfonic acid（1.5 mmol/L） as mobile phase. The flow rate was 0.8 m L/min at a column temperature of 38.5 ℃. It was found that the linearity range of betaine was 10.4–830.0 mg/L（R2 = 0.999 8） and the spiked recoveries varied in the range of 95.51%–98.06%. The proposed method was found to be simple, reproducible and suitable for the determination of betaine in L. ruthenicum.

关 键 词：离子色谱 黑果枸杞 甜菜碱 

分 类 号：O657.75[理学—分析化学]