DMBA对大鼠胰腺肿瘤发生的影响  

DMBA induces pancreatic tumorigenesis in rats

作　　者：任宇[1] 侯晓朴[2] 李丹[3,4] 李颖[3,4] 朱斌[3,4] 

机构地区：[1]首都医科大学附属北京妇产医院乳腺外科,北京市100006 [2]首都医科大学附属北京地坛医院介入科,北京市100015 [3]首都医科大学附属北京世纪坛医院普通外科 [4]北京大学第九临床医学院,北京市100038

出　　处：《世界华人消化杂志》2015年第29期4700-4705,共6页World Chinese Journal of Digestology

基　　金：铁道部科技研究开发课题基金资助项目;No.Z2011-013~~

摘　　要：目的:探讨7,1 2-二甲基苯并蒽(7,1 2-dimethylbenzanthracene,DMBA)对大鼠胰腺肿瘤发生的影响.方法:80只♂SD大鼠随机分成两组:A组(实验组)70只,将DMBA按4 mg/100 g体质量置入胰腺;B组(对照组)10只,仅行胰腺被膜的切开,不置入任何药物.2组分别在手术后每月观察体质量变化;并于手术后2、4、6 mo后取胰腺行病理组织学检查.结果:A组大鼠术后5 mo及6 mo体质量较B组明显下降.大鼠术后4 mo平滑肌肉瘤发生率为42.1%(8/19),6 mo为75%(15/20),6 mo大鼠肉瘤发生率高于4 mo大鼠肉瘤发生率(P=0.037);并伴有恶液质、血性腹水及腹腔胰腺外肿瘤.但无胰腺导管细胞癌发生.结论:采用DMBA置入SD大鼠胰腺内的方法建立胰腺癌动物模型,出现体质量增长曲线下降趋势,病理学诊断显示为急、慢性胰腺炎、平滑肌肉瘤及肿瘤浸润食管、胃及肠道等脏器,无胰腺上皮内瘤变和胰腺癌的出现.AIM：To explore the possibility of pancreatic carcinogenesis induced by7,12-dimethylbenzanthracene（DMBA）implantation in Sprague-Dawley（SD） rats.METHODS：Eighty male SD rats were randomly divided into two groups：A and B.DMBA（4 mg/ 100 g body weight） was implanted into the parenchyma of the rat pancreas in group A（n= 70）,while no drugs were implanted into the parenchyma of the pancreas in group B（n= 10）.Rats were killed at 2,4 and 6 mo after operation.The pancreatic tissue samples of rats were fixed in formalin for HE staining and immunohistochemical analysis.RESULTS：The body weight of rats at 5 and 6mo had significant differences between groups A and B（P = 0.00）.In group A,the incidence of leiomyosarcoma was 42.1%at 4 mo and75%at 6 mo,and the rats were accompanied by cachexia,bloody ascites and metastasis.The incidence of leiomyosarcoma at 6 mo was higher than that at 4 mo（P = 0.037）.No pancreatic ductal cell carcinoma occurred.CONCLUSION：DMBA（4 mg/ 100 g body weight） implantation into the parenchyma of the pancreas of SD rats induces the development of pancreatic leiomyosarcoma,and the incidence of leiomyosarcoma at6 mo is higher than that at 4 mo.DMBA implantation does not induce pancreatic ductal cell carcinoma.

关 键 词：胰腺肿瘤 平滑肌肉瘤 DMBA 动物模型 

分 类 号：R735.9[医药卫生—肿瘤]