乙肝病毒X基因对肝癌Bel7402细胞ras基因表达的影响  

The effect of hepatitis B virus X gene on the expression of ras in human hepatoma cells Bel7402

作　　者：鲁琰[1,2] 李伟[1,2] 朱明月[1,2] 董栩[1,2] 陈栘 张雪儿 李孟森[1,2,4] 

机构地区：[1]海南医学院海南省肿瘤发生和干预重点实验室,海南海口571199 [2]海南医学院分子生物学重点实验室,海南海口571199 [3]海南医学院,13卓越创新班海南海口571199 [4]海南医学院肿瘤研究所,海南海口570102

出　　处：《中国医药导报》2015年第32期9-12,共4页China Medical Herald

基　　金：国家自然科学基金(81360307;81260306;81160261;31060164);海南省社会发展科技专项(2015SF03);海南医学院科研培育基金(HY2013-19);海南医学院大学生创新创业训练计划项目(HYCX2014031)

摘　　要：目的探讨乙肝病毒X基因(HBx)对人肝癌细胞原癌基因ras表达水平的影响。方法将pcDNA3.1载体与HBx基因连接构建真核表达载体pcDNA3.1-HBx,采用脂质体介导转染人肝癌细胞Bel7402,以转染空载体pcDNA3.1的Bel7402细胞为对照,于转染后2、4、8、16 h收集细胞,提取细胞总蛋白并用Western blot技术检测肝癌细胞内n-Ras蛋白表达水平。结果与转染空载体pcDNA3.1的Bel7402对照组相比,转染重组表达载体pcDNA3.1-HBx的Bel7402细胞Ras蛋白表达水平在第2小时明显降低,第4小时Ras蛋白的表达水平仍有降低趋势,但比第2小时表达量有所增加;第8小时和第16小时,Ras蛋白的表达水平与对照组相比明显升高,差异有统计学意义(P<0.05)。结论 HBx对人肝癌Bel7402细胞Ras蛋白表达有明显影响,HBx持续表达可能对Ras表达有促进作用。Objective To investigate the influence of HBx on proto-oncogene ras expression in human hepatoma cells.Methods HBx linked to pc DNA3.1 for constructing eukaryotic expression vector pc DNA3.1-HBx. pc DNA3.1-HBx vectors were transfected into Bel7402 cells to establish Bel7402/HBx cell model. Bel7402 cells transfected with pc DNA3.1 was used as control. Western blot was applied to evaluate the expression levels of n-Ras in Bel7402 cell while transfected with pc DNA3.1-HBx vectors for 2, 4, 8, 16 h. Results The expression of Ras in Bel7402 was significantly lower while transfected with pc DNA3.1-HBx vectors for 2 h and 4 h contrast with control group（vehicle vector）, however, the expression of Ras was significantly promoted while transfected with pc DNA3.1-HBx vectors for 8 h and 16 h（P〈0.05）. Conclusion HBx plays a role in influencing the expression of Ras in Bel7402 cells. Persistent expression of HBx maybe stimulate expression of Ras in hepatoma cells.

关 键 词：肝癌 乙肝病毒X基因 RAS 

分 类 号：R734[医药卫生—肿瘤]