盐渍白萝卜的真菌菌群结构DGGE分析及酵母菌分离鉴定  

DGGE Analysis of Fungi Community Structure,Isolation and Identification of Yeasts from Salted Raphanus sativus

作　　者：尹礼国[1,2,3] 马伟玲[2] 李文芳[2] 魏劲松[1] 张其圣[2,4,5] 罗青春[2] 陈功[4,5] 吴正云[2] 张文学[2] 

机构地区：[1]宜宾学院生命科学与食品工程学院,四川宜宾644007 [2]四川大学轻纺与食品学院,成都610065 [3]宜宾学院固态发酵资源利用四川省重点实验室,四川宜宾644007 [4]四川省食品发酵工业研究设计院,成都611130 [5]四川东坡中国泡菜产业技术研究院,四川眉山620000

出　　处：《中国调味品》2015年第11期14-18,共5页China Condiment

基　　金："十二五"国家科技支撑项目(2012BAD31B04);"十二五"四川省科技支撑项目(2012NZ0002);四川省教育厅重点项目(09ZC050);发酵资源与应用四川省高校重点实验室开放基金项目(2009KFJ003);宜宾学院科研项目(2013YY001)

摘　　要：采用变性梯度凝胶电泳技术(denatured gradient gel electrophoresis,DGGE)对不同盐渍白萝卜样品的真菌菌群结构分析表明,Candida lactis-condensi、Candida versatilis、膜醭毕赤酵母(Pichia membranifaciens)、汉逊德巴氏酵母菌(Debaryomyces hansenii)为盐渍萝卜的优势菌。通过传统分离培养技术从样品中分离到17株酵母菌,通过分子生物学鉴定为Pichia sp.、膜醭毕赤酵母(Pichia membranefaciens)、德巴利酵母属(Debaryomyces sp.)。传统分离培养技术未分离到Candida lactis-condensi,Candida versatilis。DGGE技术能够快速高效、直观地反映盐渍白萝卜的菌群结构,结果合理。The fungi communities of different salted Raphanus sativus are analyzed by denatured gradient gel electrophoresis （DGGE ）.The dominant fungi groups of salted Raphanus sativus are Candida lactis-condensi,Candida versatilis,Candida sp.,Pichi sp.,the closest species are Candida lactis-condensi,Candida versatilis,Pichia membranifaciens,Debaryomyces hansenii.17 yeasts are isolated from salted Raphanus sativus by cultivation methods,and are identified by molecular biology methods,the strains are Pichi sp.,Pichia membranefaciens,and Debaryomyces hansenii.Candida lactis-condensi and Candida versatilis are not isolated with the cultivation methods.DGGE is a suitable tool for the analysis of fungi communities of salted Raphanus sativus,as it is more rapid and comprehensive than cultivation methods detection of species.

关 键 词：变性梯度凝胶电泳(DGGE) 盐渍白萝卜 酵母菌 真菌群落结构 

分 类 号：TS255.54[轻工技术与工程—农产品加工及贮藏工程]