响应面优化木立芦荟皮多糖的研究  

Optimization for Extraction of Polysaccharide from Skin of Aloe arborescens Mill.Using Response Surface Method

作　　者：谌素华[1] 邓敏[2] 王维民[1] 李国伟[1] 钟赛意[1] 胡武[1] 

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]广东海洋大学医院,广东湛江524088

出　　处：《食品研究与开发》2015年第18期154-158,共5页Food Research and Development

基　　金：2010年度湛江市科技攻关项目(湛科[2010]78号文);项目编号:2010C3103022

摘　　要：采用响应面分析法对木立芦荟皮多糖的提取工艺进行优化,对提取率影响因素进行单因素分析后,以BoxBehnken设计评价微波时间、提取液p H和液料比3个因素显著性和交互作用,最终得出木立芦荟皮多糖的最佳提取条件为:微波时间为168 s,提取液的p H为9,液料比为52∶1(m L/g),微波功率为320 W,在此条件下提取率为14.37%;并对提取的粗多糖进行红外光谱分析,结果表明木立芦荟皮粗多糖具有多糖的特征吸收峰。The extraction technology of polysaccharide from skin slag of Aloe arborescens Mill. was studied using response Surface Method（RSM）,and microwave time, Extraction liquid pH, liquid-solid ratio were selected as experimental the factors. Based on the analysis of significance and interaction among different factors, the optimal condition of extraction technology was obtained as followed：microwave time was 168 s,Extraction liquid pH was 9, liquid-solid ratio was 52 ∶ 1（mL/g）, microwave power was 320 W. Under the conditions of extraction, the average value of three times actual measured extraction rate was 14.37 %. Using IR to analyze structure of polysaccharide from skin slag of Aloe arborescens Mill, IR spectra suggested its had polysaccharide′s characteristic groups.

关 键 词：木立芦荟皮 多糖 微波辅助提取 响应面分析 红外光谱分析 

分 类 号：TQ464.1[化学工程—制药化工]