芝麻显性核不育系花蕾发育过程中游离氨基酸的变化  

Changes of Free Amino Acids in Developing Buds of a Dominant Male Sterile Line in Sesame

作　　者：刘红艳[1] 杨敏敏[1] 赵应忠[1] 

机构地区：[1]中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《核农学报》2015年第11期2071-2076,共6页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31101180);国家芝麻产业技术体系(CARS-15)

摘　　要：为探明芝麻显性细胞核雄性不育系W1098A发育过程中花药败育的生理生化机制,利用酸水解法分析芝麻显性细胞核雄性不育花蕾与正常可育花蕾发育过程中20种游离氨基酸含量的动态变化。结果表明,在花蕾发育过程中大部分氨基酸含量都是不育低于可育,表明氨基酸缺乏可能是导致花粉败育的重要原因;花蕾中主要的游离氨基酸为天冬氨酸、苏氨酸、谷氨酸、赖氨酸、脯氨酸、精氨酸,占氨基酸总含量的70%以上,其中脯氨酸含量在四分体期的不育花蕾和可育花蕾中差异十分明显,可能是造成花粉败育的主要因素。本研究阐明了芝麻雄性不育的生理生化基础,为芝麻显性雄性不育败育机理研究提供了科学依据。To study the biochemical basis of a newly developed dominant genic male sterile line W1098 A in sesame（ Sesamumindicum L.）,acid hydrolysis method was employed to characterize the dynamics of free amino acid contents in sterile and fertile buds of W1098 AB at different developmental stages. The results indicated that there were 20 types of free amino acid in buds,and that the contents of most amino acid were lower in sterile buds than in fertile buds during development,suggesting that the shortage of amino acids was associated with the occurrence of male sterility in sesame.The major free amino acids were found to be Aspartate,Threonine,Glutamate,Lysine,Proline and Arginine that accounted for 70% of the total content. Among these major amino acids,the content of Proline was remarkably lower in sterile buds than in fertile buds at tetrad stage,which might be responsible for the male sterility in W1098 A.

关 键 词：芝麻 显性核不育 花蕾 游离氨基酸 

分 类 号：S565.3[农业科学—作物学]